-
- Comment in:
-
Nature. 1992 Aug 20;358(6388):625-6.
Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides.
Waksman G,
Kominos D,
Robertson SC,
Pant N,
Baltimore D,
Birge RB,
Cowburn D,
Hanafusa H,
Mayer BJ,
Overduin M, et al.
Rockefeller University, New York.
Three-dimensional structures of complexes of the SH2 domain of the v-src oncogene product with two phosphotyrosyl peptides have been determined by X-ray crystallography at resolutions of 1.5 and 2.0 A, respectively. A central antiparallel beta-sheet in the structure is flanked by two alpha-helices, with peptide binding mediated by the sheet, intervening loops and one of the helices. The specific recognition of phosphotyrosine involves amino-aromatic interactions between lysine and arginine side chains and the ring system in addition to hydrogen-bonding interactions with the phosphate.
PMID: 1379696 [PubMed - indexed for MEDLINE]
-
Related Articles