Abstract
A cDNA of 1650 base pairs was isolated by screening an HL-60 granulocyte library with an N-formyl peptide receptor (NFPR) cDNA probe under low stringency conditions. The cDNA encodes a protein of 351 amino acids tentatively named FPR2, with a calculated molecular weight of 39 kDa. Sequence analysis revealed that FPR2 is 69% identical in sequence to the human NFPR and shares extensive homology to several other chemoattractant receptors. FPR2 expressed in transfected cells mediated formyl peptide-stimulated calcium mobilization at micromolar concentrations of ligand. FPR2 messenger is detected in granulocytic HL-60 cells, but not in undifferentiated HL-60 cells. These findings suggest that FPR2 is a novel receptor for formyl peptide ligand and a new member of the chemoattractant receptor gene family.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line
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Cloning, Molecular
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DNA Probes
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DNA, Neoplasm / genetics*
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DNA, Neoplasm / isolation & purification
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Granulocytes / physiology*
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Humans
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Leukemia, Promyelocytic, Acute
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Mice
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Molecular Sequence Data
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Molecular Weight
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Multigene Family
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N-Formylmethionine Leucyl-Phenylalanine / metabolism
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Poly A / genetics
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Poly A / isolation & purification
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Polymerase Chain Reaction / methods
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Protein Conformation
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RNA / genetics
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RNA / isolation & purification
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RNA, Messenger
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Receptors, Formyl Peptide
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Receptors, Immunologic / genetics*
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Receptors, Immunologic / metabolism
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Sequence Homology, Nucleic Acid
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Transfection
Substances
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DNA Probes
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DNA, Neoplasm
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RNA, Messenger
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Receptors, Formyl Peptide
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Receptors, Immunologic
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Poly A
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N-Formylmethionine Leucyl-Phenylalanine
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RNA
Associated data
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GENBANK/D10556
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GENBANK/M88107
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GENBANK/M97582
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GENBANK/M97583
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GENBANK/M97584
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GENBANK/M97585
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GENBANK/M97586
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GENBANK/M97587
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GENBANK/M97588
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GENBANK/M97589