Isolation of a cDNA that encodes a novel granulocyte N-formyl peptide receptor

Biochem Biophys Res Commun. 1992 Apr 30;184(2):582-9. doi: 10.1016/0006-291x(92)90629-y.

Abstract

A cDNA of 1650 base pairs was isolated by screening an HL-60 granulocyte library with an N-formyl peptide receptor (NFPR) cDNA probe under low stringency conditions. The cDNA encodes a protein of 351 amino acids tentatively named FPR2, with a calculated molecular weight of 39 kDa. Sequence analysis revealed that FPR2 is 69% identical in sequence to the human NFPR and shares extensive homology to several other chemoattractant receptors. FPR2 expressed in transfected cells mediated formyl peptide-stimulated calcium mobilization at micromolar concentrations of ligand. FPR2 messenger is detected in granulocytic HL-60 cells, but not in undifferentiated HL-60 cells. These findings suggest that FPR2 is a novel receptor for formyl peptide ligand and a new member of the chemoattractant receptor gene family.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA Probes
  • DNA, Neoplasm / genetics*
  • DNA, Neoplasm / isolation & purification
  • Granulocytes / physiology*
  • Humans
  • Leukemia, Promyelocytic, Acute
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Multigene Family
  • N-Formylmethionine Leucyl-Phenylalanine / metabolism
  • Poly A / genetics
  • Poly A / isolation & purification
  • Polymerase Chain Reaction / methods
  • Protein Conformation
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger
  • Receptors, Formyl Peptide
  • Receptors, Immunologic / genetics*
  • Receptors, Immunologic / metabolism
  • Sequence Homology, Nucleic Acid
  • Transfection

Substances

  • DNA Probes
  • DNA, Neoplasm
  • RNA, Messenger
  • Receptors, Formyl Peptide
  • Receptors, Immunologic
  • Poly A
  • N-Formylmethionine Leucyl-Phenylalanine
  • RNA

Associated data

  • GENBANK/D10556
  • GENBANK/M88107
  • GENBANK/M97582
  • GENBANK/M97583
  • GENBANK/M97584
  • GENBANK/M97585
  • GENBANK/M97586
  • GENBANK/M97587
  • GENBANK/M97588
  • GENBANK/M97589