Abstract
Human cDNA clones encoding Pit-1/GHF-1, a pituitary-specific DNA binding factor, were obtained by PCR following reverse transcription of human pituitary RNA. It is approx. 1.3 kb in size with 0.1 kb 5' non-coding region, 0.9 kb protein-coding region and 0.3 kb 3' non-coding region. The predicted human Pit-1/GHF-1 peptide structure has 291 amino acids and is highly conserved among mouse, rat and bovine. In addition, the 5' non-coding region is highly conserved with rat pit-1/GHF-1 sequence to the transcription start site.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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DNA / chemistry
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DNA-Binding Proteins / genetics*
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Humans
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Molecular Sequence Data
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Pituitary Gland / chemistry
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Polymerase Chain Reaction
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RNA / genetics
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RNA / isolation & purification
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Transcription Factor Pit-1
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Transcription Factors / genetics*
Substances
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DNA-Binding Proteins
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POU1F1 protein, human
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Transcription Factor Pit-1
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Transcription Factors
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RNA
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DNA
Associated data
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GENBANK/D01114
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GENBANK/D10216
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GENBANK/D11333
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GENBANK/D11334
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GENBANK/M91424
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GENBANK/M91425
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GENBANK/M91426
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GENBANK/M91427
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GENBANK/M91428
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GENBANK/M91429
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GENBANK/M91430