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1: Biosci Biotechnol Biochem. 1992 Mar;56(3):432-6.Links

Molecular cloning and expression of a Streptomyces sarcosine oxidase gene in Streptomyces lividans.

Suzuki K, Ogishima M, Sugiyama M, Inouye Y, Nakamura S, Imamura S.

Research Laboratories, Toyo Jozo Co., Ltd., Shizuoka, Japan.

A genomic library of Streptomyces sp. KB210-8SY, prepared in the plasmid vector pACYC184, was screened to obtain the gene encoding sarcosine oxidase with probes based on the amino acid sequence of the protein. A plasmid pSOXS13, which was isolated from a clone identified by hybridization with the probes, contained a 8.4-kb insert of Streptomyces DNA. When the 2.0-kb MIuI/EcoRV DNA fragment of pSOXS13 was inserted into the Streptomyces vector pIJ680 and introduced into S. lividans, the transformants produced 100-fold more sarcosine oxidase intracellularly than KB210-8SY. The nucleotides of the 1.7-kb fragment containing the sarcosine oxidase gene were sequenced. An open reading frame encoded a mature sarcosine oxidase consisting of 388 amino acids, with a calculated molecular mass of 42,107 daltons.

PMID: 1368326 [PubMed - indexed for MEDLINE]

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