Assignment of genes encoding a unique cytokine (IL12) composed of two unrelated subunits to chromosomes 3 and 5

Genomics. 1992 Sep;14(1):59-62. doi: 10.1016/s0888-7543(05)80283-6.

Abstract

IL12 (formerly NKSF or CLMF) is a unique cytokine composed of two unrelated disulfide-linked subunits. The larger 40-kDa subunit (p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit (p35) is related to IL6 and GCSF. The chromosomal localization of these two subunits was determined by PCR analysis of DNA from rodent-human hybrids. More refined mapping was obtained by PCR analysis of hybrids containing translocation chromosomes and for p40, by analysis of radiation hybrids. The subunits map to different chromosomes: p40 (IL12B) to 5q31-q33 and p35 (IL12A) to 3p12-3q13.2.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Southern
  • Chromosomes, Human, Pair 3*
  • Chromosomes, Human, Pair 5*
  • Cricetinae
  • DNA / analysis
  • Humans
  • Hybrid Cells
  • Interleukin-12
  • Interleukins / genetics*
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Polymerase Chain Reaction

Substances

  • Interleukins
  • Oligonucleotide Probes
  • Interleukin-12
  • DNA