An interaction between p21ras and heat shock protein hsp60, a chaperonin

Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2012-6. doi: 10.1073/pnas.89.6.2012.

Abstract

Ras proteins play a crucial role in the development of neoplasia and in signal transduction in normal cells. In a search for proteins interacting with p21ras, we previously identified a protein of 60 kDa (p60) through use of a chemical cross-linker. Using information from partial amino acid sequencing of the purified protein, we isolated full-length cDNA clones encoding this 60-kDa protein. Nucleotide sequence analysis revealed that p60 is the murine heat shock protein hsp60, a chaperonin. Association of hsp60 with p21ras appears physiological, as the amount of hsp60 complexed to p21ras was similar even in cells over-expressing p21ras, and reversing the order of cross-linking and lysis of the cells, which releases large amounts of hsp60 from mitochondria, did not alter the amount of hsp60 cross-linked to p21ras.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Base Sequence
  • Cell Line
  • Chaperonins
  • Cloning, Molecular
  • Cross-Linking Reagents
  • Heat-Shock Proteins / genetics
  • Heat-Shock Proteins / isolation & purification
  • Heat-Shock Proteins / metabolism*
  • Humans
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemical synthesis
  • Proteins / genetics
  • Proteins / metabolism*
  • Proto-Oncogene Proteins p21(ras) / genetics
  • Proto-Oncogene Proteins p21(ras) / metabolism*
  • Rats
  • Sequence Homology, Nucleic Acid
  • Succinimides

Substances

  • Antibodies, Monoclonal
  • Cross-Linking Reagents
  • Heat-Shock Proteins
  • Oligodeoxyribonucleotides
  • Proteins
  • Succinimides
  • Chaperonins
  • HRAS protein, human
  • Proto-Oncogene Proteins p21(ras)
  • dithiobis(succinimidylpropionate)