Abstract
The 5'-flanking region of the human brain-derived neurotrophic factor (BDNF) gene was isolated from a human placental genomic library using the cDNA fragment for the 5'-noncoding region of human BDNF as a probe. A 3.2 Kbp genomic fragment containing the 5'-flanking region, the first exon and a portion of the first intron was isolated and sequenced. The transcriptional initiation site, identified by S1 nuclease mapping, was located 26 bp downstream from the TATA-like sequence. Several expression plasmids, in which the BDNF promoter regions were fused to the chloramphenicol acetyltransferase (CAT) gene, were constructed. Transient expression in human glioma Hs683 cells demonstrated that a fragment of about 0.5 Kbp from the transcriptional initiation site was sufficient for promoter activity.
MeSH terms
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Base Sequence
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Brain-Derived Neurotrophic Factor
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Chloramphenicol O-Acetyltransferase / genetics
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Chloramphenicol O-Acetyltransferase / metabolism
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Female
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Gene Library
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Glioma
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Humans
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Molecular Sequence Data
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Nerve Growth Factors / genetics*
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Nerve Tissue Proteins / genetics*
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Oligodeoxyribonucleotides
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Placenta / physiology
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Pregnancy
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Promoter Regions, Genetic*
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Recombinant Fusion Proteins / metabolism
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TATA Box
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Transcription, Genetic
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Transfection
Substances
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Brain-Derived Neurotrophic Factor
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Nerve Growth Factors
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Nerve Tissue Proteins
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Oligodeoxyribonucleotides
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Recombinant Fusion Proteins
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Chloramphenicol O-Acetyltransferase
Associated data
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GENBANK/D10271
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GENBANK/D10272
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GENBANK/D10273
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GENBANK/D10274
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GENBANK/D10275
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GENBANK/D10276
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GENBANK/M84512
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GENBANK/M84513
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GENBANK/S75889
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GENBANK/S75896