Abstract
Two cases of globoid cell leukodystrophy (Krabbe's disease) have been studied by means of conventional histological, immunohistochemical and ultrastructural methods. Lectins specific for several different carbohydrates, Ricinus communis (RCA-1), peanut agglutinin (PNA), wheat germ agglutinin (WGA) and Concanavalin A (Con A) have been utilized both at optical and ultrastructural level. In both cases a strong positive labeling of the globoid cells could be observed light-microscopically. Ultrastructurally immunogold deposits only of RCA-I and WGA in the typical Krabbe's inclusions have been clearly demonstrated.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylgalactosamine / analysis
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Acetylglucosamine / analysis
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Blood Glucose / analysis
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Brain / pathology*
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Cytoplasm / ultrastructure
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Galactose / analysis
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Humans
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Immunoenzyme Techniques
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Inclusion Bodies / ultrastructure
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Infant
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Leukodystrophy, Globoid Cell / genetics
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Leukodystrophy, Globoid Cell / pathology*
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Male
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Mannose / analysis
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Microscopy, Electron
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Myelin Sheath / pathology
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N-Acetylneuraminic Acid
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Neurons / pathology
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Receptors, Mitogen / analysis*
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Sialic Acids / analysis
Substances
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Blood Glucose
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Receptors, Mitogen
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Sialic Acids
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N-Acetylneuraminic Acid
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Acetylgalactosamine
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Mannose
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Acetylglucosamine
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Galactose