Abstract
We have shown that a drug-resistant mutant from a clinical isolate of herpes simplex virus contains a single point mutation in the DNA polymerase gene that confers resistance to both acyclovir and foscarnet. The mutated amino acid is located within a distinct conserved region shared among alpha-like DNA polymerases which we designate region VII. We infer that these conserved sequences are directly or indirectly involved in the recognition and binding of nucleotide and PPi substrates.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acyclovir / pharmacology
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Amino Acid Sequence
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DNA-Directed DNA Polymerase / genetics*
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Drug Resistance, Microbial
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Foscarnet
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Genes, Viral
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Molecular Sequence Data
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Mutation
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Phosphonoacetic Acid / analogs & derivatives
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Phosphonoacetic Acid / pharmacology
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Sequence Alignment
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Simplexvirus / drug effects
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Simplexvirus / enzymology
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Simplexvirus / genetics*
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Tyrosine / chemistry
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Viral Structural Proteins / genetics
Substances
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Viral Structural Proteins
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Foscarnet
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Tyrosine
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DNA-Directed DNA Polymerase
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Phosphonoacetic Acid
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Acyclovir