Cleavage of Arabidopsis PBS1 by a bacterial type III effector

Science. 2003 Aug 29;301(5637):1230-3. doi: 10.1126/science.1085671.

Abstract

Plant disease-resistance (R) proteins are thought to function as receptors for ligands produced directly or indirectly by pathogen avirulence (Avr) proteins. The biochemical functions of most Avr proteins are unknown, and the mechanisms by which they activate R proteins have not been determined. In Arabidopsis, resistance to Pseudomonas syringae strains expressing AvrPphB requires RPS5, a member of the class of R proteins that have a predicted nucleotide-binding site and leucine-rich repeats, and PBS1, a protein kinase. AvrPphB was found to proteolytically cleave PBS1, and this cleavage was required for RPS5-mediated resistance, which indicates that AvrPphB is detected indirectly via its enzymatic activity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis / microbiology
  • Arabidopsis Proteins / chemistry
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Cell Line
  • Cysteine Endopeptidases / chemistry
  • Cysteine Endopeptidases / genetics
  • Cysteine Endopeptidases / metabolism*
  • Genes, Bacterial
  • Genes, Plant
  • Genetic Complementation Test
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Models, Biological
  • Molecular Sequence Data
  • Mutation
  • Nicotiana / genetics
  • Nicotiana / metabolism
  • Phosphorylation
  • Plant Diseases / microbiology*
  • Plant Extracts / metabolism
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plants, Genetically Modified
  • Precipitin Tests
  • Protein Serine-Threonine Kinases / chemistry
  • Protein Serine-Threonine Kinases / genetics
  • Protein Serine-Threonine Kinases / metabolism*
  • Protein Structure, Tertiary
  • Pseudomonas / metabolism*
  • Recombinant Proteins / metabolism
  • Transformation, Genetic

Substances

  • Arabidopsis Proteins
  • Bacterial Proteins
  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • PBS2 protein, Arabidopsis
  • Plant Extracts
  • Plant Proteins
  • RPS5 protein, Arabidopsis
  • Recombinant Proteins
  • PBS1 protein, Arabidopsis
  • Protein Serine-Threonine Kinases
  • Cysteine Endopeptidases