Efflux of cytoplasmically acting antibiotics from gram-negative bacteria: periplasmic substrate capture by multicomponent efflux pumps inferred from their cooperative action with single-component transporters

Michael Palmer

doi:10.1128/JB.185.17.5287-5289.2003

Efflux of cytoplasmically acting antibiotics from gram-negative bacteria: periplasmic substrate capture by multicomponent efflux pumps inferred from their cooperative action with single-component transporters

J Bacteriol. 2003 Sep;185(17):5287-9. doi: 10.1128/JB.185.17.5287-5289.2003.

Author

Michael Palmer¹

Affiliation

¹ Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada. mpalmer@uwaterloo.ca

Abstract

In gram-negative bacteria, coexpression of single- and multicomponent efflux pumps may result in multiplicative enhancement of the level of resistance against cytoplasmically acting antibiotics. Here, a simple model is presented to show that this cooperative effect can be accounted for only if substrate capture by the multicomponent efflux transporter occurs in the periplasm but not the cytosol.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / metabolism*
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / metabolism
Cell Membrane / metabolism
Cytoplasm / metabolism*
Cytosol / metabolism
Drug Resistance, Bacterial
Gram-Negative Bacteria / drug effects*
Membrane Transport Proteins / metabolism*
Models, Biological*
Periplasm / metabolism*

Substances

Anti-Bacterial Agents
Bacterial Proteins
Membrane Transport Proteins