Synthesis of non-natural DNA using DNA polymerase

Nucleic Acids Symp Ser. 2000:(44):143-4. doi: 10.1093/nass/44.1.143.

Abstract

Nonnatural nucleotide modified by glucose or galactose was synthesized to increase functional diversity of DNA library. These compounds were incorporated in a DNA double strand using Klenow Fragment as well as dTTP. These functional group could be ordered sequentially on a DNA double strand at intervals of few angstroms according to the designed template sequence within a few hours. This method must be useful to constructing nonnatural DNA library or designed supramolecular structures.

MeSH terms

  • Base Sequence
  • DNA / biosynthesis*
  • DNA / chemistry*
  • DNA Polymerase I / metabolism*
  • Deoxyuracil Nucleotides / chemistry
  • Deoxyuracil Nucleotides / metabolism
  • Drug Design
  • Galactose / chemistry
  • Glucose / chemistry
  • Models, Molecular
  • Molecular Structure
  • Nucleic Acid Conformation
  • Substrate Specificity
  • Thymine Nucleotides / chemistry
  • Thymine Nucleotides / metabolism

Substances

  • Deoxyuracil Nucleotides
  • Thymine Nucleotides
  • deoxyuridine triphosphate
  • DNA
  • DNA Polymerase I
  • Glucose
  • thymidine 5'-triphosphate
  • Galactose