Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides

A Masaoka; H Terato; A Honsho; Y Ohyama; T Suzuki; M Yamada; K Makino; H Ide

doi:10.1093/nass/44.1.87

Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides

Nucleic Acids Symp Ser. 2000:(44):87-8. doi: 10.1093/nass/44.1.87.

Authors

A Masaoka¹, H Terato, A Honsho, Y Ohyama, T Suzuki, M Yamada, K Makino, H Ide

Affiliation

¹ Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.

PMID: 12903281
DOI: 10.1093/nass/44.1.87

Abstract

Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Damage*
DNA Glycosylases
DNA Repair
Deoxyribonucleotides / metabolism
Guanine / metabolism*
In Vitro Techniques
N-Glycosyl Hydrolases / metabolism
Nitric Oxide / toxicity*
Nitrogen Dioxide / toxicity*
Nucleotides / metabolism
Purine Nucleosides / metabolism
Pyrroles / metabolism
Xanthine / metabolism

Substances

1-(2'-deoxyribofuranosyl)-3-nitropyrrole triphosphate
Deoxyribonucleotides
Nucleotides
Purine Nucleosides
Pyrroles
oxanine
Xanthine
Nitric Oxide
Guanine
DNA Glycosylases
N-Glycosyl Hydrolases
Nitrogen Dioxide