Abstract
Protein-tyrosine phosphorylation and dephosphorylation are directly associated with cellular growth, signal transduction, and neoplastic transformation. Here we report the isolation of a complementary DNA (cDNA) clone encoding a novel protein-tyrosine phosphatase (PTP) from a human T cell PEER cDNA library. The predicted open reading frame encodes a approximately 68-kDa protein composed of 593 amino acids which contains two src-homology region 2's (SH2 domains) at the N terminus; this PTP is designated as SH-PTP3. Northern blot analysis revealed that SH-PTP3 mRNA was expressed throughout many tissues and the transcriptional size was consistent at about 6.0 kb. As with other SH2 domains in src-family kinases, the SH2 domains of SH-PTP3 may play a crucial role in interactions with tyrosine phosphorylated signaling proteins, including itself and protein tyrosine kinases (PTKs), to regulate targets' enzyme activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Blotting, Southern
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Cloning, Molecular
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DNA
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Humans
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Intracellular Signaling Peptides and Proteins
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Molecular Sequence Data
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Protein Tyrosine Phosphatase, Non-Receptor Type 11
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Protein Tyrosine Phosphatase, Non-Receptor Type 6
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Protein Tyrosine Phosphatases / genetics*
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Protein Tyrosine Phosphatases / metabolism
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Protein-Tyrosine Kinases / metabolism
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Proto-Oncogene Proteins pp60(c-src)*
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Rats
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Sequence Homology, Amino Acid
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Transcription, Genetic
Substances
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Intracellular Signaling Peptides and Proteins
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DNA
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Protein-Tyrosine Kinases
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Proto-Oncogene Proteins pp60(c-src)
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PTPN11 protein, human
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PTPN6 protein, human
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Protein Tyrosine Phosphatase, Non-Receptor Type 11
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Protein Tyrosine Phosphatase, Non-Receptor Type 6
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Protein Tyrosine Phosphatases
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Ptpn11 protein, rat
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Ptpn6 protein, rat
Associated data
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GENBANK/D13540
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GENBANK/S39996
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GENBANK/S40001
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GENBANK/S40008
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GENBANK/S40009
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GENBANK/S40010
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GENBANK/X72580
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GENBANK/Z18281
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GENBANK/Z18282
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GENBANK/Z18283