Multiple roles for kinases in DNA replication

EMBO Rep. 2003 Mar;4(3):252-6. doi: 10.1038/sj.embor.embor774.

Abstract

DNA replication is carried out by the replisome, which includes several proteins that are targets of cell-cycle-regulated kinases. The phosphorylation of proteins such as replication protein A, DNA polymerase-alpha and -delta, replication factor C, flap endonuclease 1 and DNA ligase I leads to their inactivation, suggesting that phosphorylation is important in the prevention of re-replication. Moreover, the phosphorylation of several of these replication proteins has been shown to block their association with the 'moving platform'-proliferating cell nuclear antigen. Therefore, phosphorylation seems to be a crucial regulator of replisome assembly and DNA replication, although its precise role in these processes remains to be clarified.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cell Cycle / physiology*
  • Cyclin-Dependent Kinases / physiology*
  • DNA Polymerase I / metabolism
  • DNA Polymerase III / metabolism
  • DNA Replication*
  • Humans
  • Phosphorylation
  • Signal Transduction / physiology

Substances

  • Cyclin-Dependent Kinases
  • DNA Polymerase I
  • DNA Polymerase III