Cutting edge: Toll-like receptor 9 expression is not required for CpG DNA-aided cross-presentation of DNA-conjugated antigens but essential for cross-priming of CD8 T cells

Antje Heit; Tobias Maurer; Hubertus Hochrein; Stefan Bauer; Katharina M Huster; Dirk H Busch; Hermann Wagner

doi:10.4049/jimmunol.170.6.2802

Cutting edge: Toll-like receptor 9 expression is not required for CpG DNA-aided cross-presentation of DNA-conjugated antigens but essential for cross-priming of CD8 T cells

J Immunol. 2003 Mar 15;170(6):2802-5. doi: 10.4049/jimmunol.170.6.2802.

Authors

Antje Heit¹, Tobias Maurer, Hubertus Hochrein, Stefan Bauer, Katharina M Huster, Dirk H Busch, Hermann Wagner

Affiliation

¹ Institute of Medical Microbiology, Immunology and Hygiene, Technische Universität München, Munich, Germany.

PMID: 12626528
DOI: 10.4049/jimmunol.170.6.2802

Abstract

Covalent linkage of immunostimulatory CpG DNA to OVA results in CpG DNA-aided cross-presentation of OVA by dendritic cells (DCs). In vivo, cross-presentation is conditional for cross-priming of OVA-specific CD8 T cells. In this study, we investigated the involvement of the CpG DNA receptor Toll-like receptor (TLR)9 in CpG DNA-aided cross-presentation and cross-priming. Although CpG DNA-aided cross-presentation is not altered in TLR9-deficient cells, TLR9 is required for maturation of APC allowing cross-priming, as resulting in CTL function. These findings imply that TLR9 does not trigger endocytosis of CpG-OVA conjugates, but activates DCs downstream of endocytosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic / metabolism
Adjuvants, Immunologic / physiology*
Animals
Antigen Presentation*
Antigen-Presenting Cells / immunology
Antigen-Presenting Cells / metabolism
CD8-Positive T-Lymphocytes / immunology*
Cell Line
Cells, Cultured
CpG Islands / immunology
DNA / immunology*
DNA / metabolism
DNA-Binding Proteins / biosynthesis*
DNA-Binding Proteins / deficiency
DNA-Binding Proteins / genetics
DNA-Binding Proteins / physiology
Dendritic Cells / immunology
Dendritic Cells / metabolism
Endocytosis / immunology
Immunization
Immunoconjugates / immunology*
Immunoconjugates / metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Oligodeoxyribonucleotides / immunology*
Oligodeoxyribonucleotides / metabolism
Ovalbumin / immunology*
Ovalbumin / metabolism
Receptors, Cell Surface / biosynthesis*
Receptors, Cell Surface / deficiency
Receptors, Cell Surface / genetics
Receptors, Cell Surface / physiology
T-Lymphocytes, Cytotoxic / immunology
T-Lymphocytes, Cytotoxic / metabolism
Toll-Like Receptor 9

Substances

Adjuvants, Immunologic
CPG-oligonucleotide
DNA-Binding Proteins
Immunoconjugates
Oligodeoxyribonucleotides
Receptors, Cell Surface
Tlr9 protein, mouse
Toll-Like Receptor 9
Ovalbumin
DNA