Abstract
Based on homology analysis and RT-PCR, a novel isoform of human TCP11 gene was isolated. It encodes a 503 amino acid protein that is highly homologous to the mouse 566 amino acid protein Tcp-11. Tcp-11 is important to sperm function because it may be the receptor of fertilization promoting peptide (FPP). Complicated alternative splicing was found to exist between TCP11a and TCP11b genes. The gene has been mapped to human chromosome band 6p21 by fluorescence in situ hybridization. Results of Northern blot and RT-PCR analysis of RNA extracted from human tissues revealed that the gene was expressed in fertile adult testes only, but neither in azoospermic patient testes, fetal testes nor in other human tissues. Our results suggest that TCP11b gene may be important to human sperm function and male fertility.
MeSH terms
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Alternative Splicing*
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Chromosome Mapping
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Chromosomes, Human, Pair 6 / genetics
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Cloning, Molecular
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Female
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Gene Expression
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Humans
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In Situ Hybridization, Fluorescence
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Intracellular Signaling Peptides and Proteins*
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Male
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Membrane Proteins
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Microtubule-Associated Proteins / genetics*
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Molecular Sequence Data
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Nuclear Proteins / genetics*
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Protein Isoforms / genetics
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Testicular Hormones / genetics
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Ubiquitin-Protein Ligases
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t-Complex Genome Region
Substances
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DNA, Complementary
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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Microtubule-Associated Proteins
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Nuclear Proteins
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Protein Isoforms
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RNA, Messenger
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TCP11 protein, human
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Tcp11 protein, mouse
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Testicular Hormones
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PPP1R11 protein, human
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Ubiquitin-Protein Ligases