Binding of the human homolog of the Drosophila discs large tumor suppressor protein to the mitochondrial ribosomal protein MRP-S34

Biochem Biophys Res Commun. 2003 Jan 17;300(3):789-92. doi: 10.1016/s0006-291x(02)02887-5.

Abstract

The human homolog of the Drosophila discs large tumor suppressor protein (hDLG) functions as a scaffolding protein that facilitates the transmission of diverse downstream signals. Here we show that hDLG interacts through its PDZ domains with the carboxy-terminal S/TXV motif of the mitochondrial ribosomal protein S-34 (MRP-S34). Our results suggest that hDLG interacts with MRP-S34 prior to entry of MRP-S34 into the mitochondria and may be involved in the trafficking of MRP-S34.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Discs Large Homolog 1 Protein
  • Drosophila
  • Drosophila Proteins*
  • Humans
  • Immunoblotting
  • Insect Proteins / genetics*
  • Membrane Proteins
  • Mitochondrial Proteins / genetics
  • Mitochondrial Proteins / metabolism*
  • Molecular Sequence Data
  • Precipitin Tests
  • Protein Binding / physiology
  • Proteins / genetics
  • Proteins / metabolism*
  • Ribosomal Proteins / genetics
  • Ribosomal Proteins / metabolism*
  • Sequence Homology, Amino Acid
  • Tumor Suppressor Proteins / genetics*
  • Tumor Suppressor Proteins / metabolism*
  • Two-Hybrid System Techniques

Substances

  • Adaptor Proteins, Signal Transducing
  • DLG1 protein, human
  • Discs Large Homolog 1 Protein
  • Drosophila Proteins
  • Insect Proteins
  • MRPS34 protein, human
  • Membrane Proteins
  • Mitochondrial Proteins
  • Proteins
  • Ribosomal Proteins
  • Tumor Suppressor Proteins
  • dlg1 protein, Drosophila