The utility of green fluorescent protein in transgenic plants

Plant Cell Rep. 2001 Jul;20(5):376-82. doi: 10.1007/s002990100346.

Abstract

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has proven to be a powerful tool in plant genetic transformation studies. This paper reviews the history and the progression of the expression of GFP variants in transgenic plants. The distinguishing features of the most useful GFPs, such as those including the S65T chromophore mutation and those with dual excitation peaks, are discussed. The review also focuses on the utility of GFP as a visual selectable marker in aiding the plant transformation process; GFP has been more important in monocot transformation compared with dicot transformation. Finally, the potential utility of new fluorescent proteins is speculated upon.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • Cnidaria
  • Gene Expression Regulation, Plant*
  • Genes, Reporter
  • Green Fluorescent Proteins
  • Luminescent Proteins / genetics*
  • Mutagenesis, Site-Directed
  • Mutation
  • Plants / genetics*
  • Plants, Genetically Modified*

Substances

  • Luminescent Proteins
  • Green Fluorescent Proteins