Cloning and characterization of a novel synaptosome-enriched mRNA that encodes 31 kDa protein

Biochim Biophys Acta. 2002 Dec 12;1579(2-3):189-95. doi: 10.1016/s0167-4781(02)00538-9.

Abstract

Although a subpopulation of mRNAs has been identified as translocated to the dendrites or the synaptic regions of neurons, the translocational mechanism has not been elucidated. To find mRNAs enriched in synapses, we compared the synaptosomal mRNAs with those from whole forebrain using differential display (DD). We cloned one of these mRNAs, which encoded a novel 31 kDa protein (PMES-2). PMES-2 mRNA was specifically transcribed in the brain and was present in the dendrites of the hippocampal neurons. PMES-2 protein was partly localized in the postsynaptic density. Although this protein is very similar to human NABC1 protein, its function is still unknown.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • COS Cells / metabolism
  • Cloning, Molecular
  • DNA, Complementary / biosynthesis
  • DNA, Complementary / chemistry
  • Dendrites / metabolism
  • Escherichia coli
  • Gene Expression Profiling
  • Immunoblotting
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / isolation & purification
  • Nerve Tissue Proteins / metabolism
  • Polymerase Chain Reaction
  • Prosencephalon / metabolism*
  • RNA / isolation & purification
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics*
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Alignment
  • Synaptosomes / metabolism*

Substances

  • BCAS1 protein, human
  • Bcas1 protein, rat
  • DNA, Complementary
  • Neoplasm Proteins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • RNA

Associated data

  • GENBANK/AB029897