Motors and switches: AAA+ machines within the replisome

Megan J Davey; David Jeruzalmi; John Kuriyan; Mike O'Donnell

doi:10.1038/nrm949

Motors and switches: AAA+ machines within the replisome

Nat Rev Mol Cell Biol. 2002 Nov;3(11):826-35. doi: 10.1038/nrm949.

Authors

Megan J Davey¹, David Jeruzalmi, John Kuriyan, Mike O'Donnell

Affiliation

¹ Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.

PMID: 12415300
DOI: 10.1038/nrm949

Abstract

Clamp loaders are required to load the ring-shaped clamps that tether replicative DNA polymerases onto DNA. Recently solved crystal structures, along with a series of biochemical studies, have provided a detailed understanding of the clamp loading reaction. In particular, studies of the Escherichia coli clamp loader--an AAA+ machine--have provided insights into the architecture of clamp loaders from eukaryotes, bacteriophage T4 and archaea. Other AAA+ proteins are also involved in the initiation of DNA replication, and studies of the E. coli clamp loader indicate mechanisms by which these proteins might function.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Review

MeSH terms

Archaea / physiology
Bacterial Proteins / physiology
Bacteriophages / physiology
DNA Replication / physiology*
DNA-Binding Proteins / chemistry
DNA-Binding Proteins / physiology*
DNA-Directed DNA Polymerase / chemistry
DNA-Directed DNA Polymerase / physiology
Escherichia coli / physiology
Eukaryotic Cells / physiology
Humans
Molecular Motor Proteins / physiology*
Origin Recognition Complex
Protein Structure, Tertiary

Substances

Bacterial Proteins
DNA-Binding Proteins
DnaA protein, Bacteria
Molecular Motor Proteins
Origin Recognition Complex
DNA-Directed DNA Polymerase