Control of complex formation of DNA polymerase alpha-primase and cell-free DNA replication by the C-terminal amino acids of the largest subunit p180

R W P Smith; H-P Nasheuer

doi:10.1016/s0014-5793(02)03197-6

Control of complex formation of DNA polymerase alpha-primase and cell-free DNA replication by the C-terminal amino acids of the largest subunit p180

FEBS Lett. 2002 Sep 11;527(1-3):143-6. doi: 10.1016/s0014-5793(02)03197-6.

Authors

R W P Smith¹, H-P Nasheuer

Affiliation

¹ Abteilung Biochemie, Institut für Molekulare Biotechnologie, Abteilung Biochemie, Beutenbergstr. 11, D-07745, Jena, Germany

PMID: 12220650
DOI: 10.1016/s0014-5793(02)03197-6

Abstract

DNA polymerase alpha-primase is a heterotetrameric complex essential for simian vacuolating virus 40 (SV40) DNA replication. We show that the C-terminal 67 amino acid residues of the human p180 subunit are essential for SV40 DNA replication as they are required for binding of the p68 subunit and play a role in the interaction with the primase subunits, p48 and p58. Furthermore, we demonstrate that exchanging these residues to those of mouse origin can only partially rescue the SV40 DNA replication activity of DNA polymerase alpha-primase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell-Free System
DNA Polymerase I / genetics
DNA Polymerase I / metabolism*
DNA Primase / genetics
DNA Primase / metabolism*
DNA Replication*
Humans
Mice
Protein Subunits
Sequence Deletion
Simian virus 40 / genetics

Substances

Protein Subunits
DNA Primase
DNA polymerase alpha-primase
DNA Polymerase I