Cloning of mouse genomic ribosomal protein L6 gene and analysis of its promoter

Biochim Biophys Acta. 2002 Jun 7;1576(1-2):219-24. doi: 10.1016/s0167-4781(02)00340-8.

Abstract

Ribosomal protein (Rp) L6 is also defined as Taxreb107 (Tax responsive element binding protein 107) for its binding activity to the long terminal repeats of human T cell leukemia virus (HTLV)-I. We cloned the genomic gene of mouse RpL6/Taxreb107 and analyzed its exon/intron structures. The promoter of RpL6/Taxreb107 contains recognition sites for multiple transcription factors including nuclear factor (NF)-kappa B. Luciferase reporter assay showed that the RpL6/Taxreb107 promoter has a constitutive activity in transfected cells, and the constitutive activity depends on the intact promoter. Expression of HTLV-I viral protein Tax mildly but reproducibly induced RpL6/Taxreb107 mRNA and promoter activity. We provide evidence suggesting that induction of RpL6/Taxreb107 by Tax is at least partially mediated by the NF-kappa B site in the promoter of RpL6/Taxreb107. Taken together, Tax up-regulates RpL6/Taxreb107 and this may provide a feedback mechanism to facilitate proliferation of HTLV-I-infected cells and production of viral particles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COS Cells
  • Cloning, Molecular
  • DNA, Complementary / biosynthesis
  • DNA, Complementary / chemistry
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Embryo, Mammalian / metabolism
  • Gene Expression Regulation, Developmental
  • Gene Products, tax / metabolism
  • Mice
  • NF-kappa B / chemistry
  • NF-kappa B / metabolism
  • Promoter Regions, Genetic*
  • Ribosomal Proteins / chemistry
  • Ribosomal Proteins / genetics*
  • Ribosomal Proteins / metabolism
  • Transfection

Substances

  • DNA, Complementary
  • DNA-Binding Proteins
  • Gene Products, tax
  • NF-kappa B
  • Ribosomal Proteins
  • ribosomal protein L6
  • tax-responsive-element-binding protein 107, mouse