Protein inhibitor of neuronal nitric oxide synthase interacts with protein kinase A inhibitors

Jianqiang Yu; Long Yu; Zheng Chen; Lihua Zheng; Xiaosong Chen; Xiang Wang; Daming Ren; Shouyuan Zhao

doi:10.1016/s0169-328x(02)00104-3

Protein inhibitor of neuronal nitric oxide synthase interacts with protein kinase A inhibitors

Brain Res Mol Brain Res. 2002 Mar 28;99(2):145-9. doi: 10.1016/s0169-328x(02)00104-3.

Authors

Jianqiang Yu¹, Long Yu, Zheng Chen, Lihua Zheng, Xiaosong Chen, Xiang Wang, Daming Ren, Shouyuan Zhao

Affiliation

¹ State Key Laboratory of Genetics Engineering, Institute of Genetics, School of Life Science, Fudan University, 220 Handan Road, Shanghai 200433, PR China.

PMID: 11978406
DOI: 10.1016/s0169-328x(02)00104-3

Abstract

Protein kinase A (PKA) and neuronal nitric oxide synthase (nNOS) are important signaling molecules. It is well known that PKA can specifically phosphorylate nNOS. But the underlying molecular mechanism is still obscure. Our data indicate that the protein inhibitor of nNOS (PIN) binds to protein kinase A inhibitors (PKIs), which suggests that PKIs, together with PIN, might mediate the phosphorylation of nNOS by PKA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carrier Proteins / metabolism*
Cells, Cultured
Central Nervous System / enzymology*
Cyclic AMP-Dependent Protein Kinases / metabolism*
Drosophila Proteins*
Dyneins
Genes, Reporter / genetics
Humans
Intracellular Signaling Peptides and Proteins*
Nitrergic Neurons / enzymology*
Nitric Oxide / metabolism*
Nitric Oxide Synthase / metabolism*
Phosphorylation
Signal Transduction / genetics
Synaptic Transmission / physiology*
Two-Hybrid System Techniques
Yeasts / enzymology

Substances

Carrier Proteins
Drosophila Proteins
Intracellular Signaling Peptides and Proteins
protein kinase modulator
Nitric Oxide
Nitric Oxide Synthase
Cyclic AMP-Dependent Protein Kinases
Dyneins