ik3-2, a relative to ik3-1/cables, is associated with cdk3, cdk5, and c-abl

Biochim Biophys Acta. 2002 Mar 19;1574(2):157-63. doi: 10.1016/s0167-4781(01)00367-0.

Abstract

A cDNA coding for ik3-2 (designated as ik3-2 from an interactor-2 with cdk3) was cloned by cross-hybridization with ik3-1 and RT-PCR. Analysis of amino acid sequence indicated that ik3-2 has the C-terminal cyclin-box-like region highly homologous to that of ik3-1 (identity in amino acids: 78%). On the other hand, the remainder of ik3-2 gene is not so similar to that of ik3-1. There are several regions other than the C-terminal cyclin-box-like region that are conserved between ik3-1 and ik3-2. In vivo binding assay indicated that like ik3-1, ik3-2 binds to cdk3, cdk5, and c-abl, although ik3-2 binds to cdk3 weakly as compared with ik3-1. The C-terminal cyclin-box-like region of ik3-2 (123 amino acids) is able to be associated with cdk5. Accordingly, ik3-2 is very similar to ik3-1 concerning its molecular interaction with other molecules, suggesting that ik3-2 function in the same biological field as ik3-1. Northern blot analysis indicated that ik3-2 is expressed ubiquitously all over tissues.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Brain / metabolism
  • COS Cells
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cell Cycle Proteins
  • Cloning, Molecular
  • Cyclin-Dependent Kinase 3
  • Cyclin-Dependent Kinase 5
  • Cyclin-Dependent Kinases / metabolism
  • Cyclins / chemistry
  • Cyclins / genetics*
  • Cyclins / metabolism
  • DNA, Complementary / biosynthesis
  • DNA, Complementary / chemistry
  • Mice
  • Molecular Sequence Data
  • Phosphoproteins*
  • Protein Binding
  • Proto-Oncogene Proteins c-abl / metabolism
  • Transfection

Substances

  • CABLES1 protein, human
  • Cables1 protein, mouse
  • Cables2 protein, mouse
  • Carrier Proteins
  • Cell Cycle Proteins
  • Cyclins
  • DNA, Complementary
  • Phosphoproteins
  • Proto-Oncogene Proteins c-abl
  • Cyclin-Dependent Kinase 5
  • Cdk3 protein, mouse
  • Cdk5 protein, mouse
  • Cyclin-Dependent Kinase 3
  • Cyclin-Dependent Kinases