The actin-binding protein Filamin-A interacts with the metabotropic glutamate receptor type 7

FEBS Lett. 2002 Mar 13;514(2-3):184-8. doi: 10.1016/s0014-5793(02)02361-x.

Abstract

A yeast two-hybrid screen identified Filamin-A as a binding partner of the metabotropic glutamate receptor type 7b (mGluR7b) splice variant. In addition, Filamin-A interacted with mGluR4a, mGluR5a, mGluR5b, mGluR7a and mGluR8a. Domain mapping revealed that alternative splicing of mGluR4, mGluR7 and mGluR8 C-termini regulated the interaction. A conserved tyrosine within mGluR C-termini was identified to mediate the binding to Filamin-A. Protein interactions were verified in biochemical assays using recombinant and native proteins. Finally, co-expression of Filamin-A and mGluR7 splice variants was shown in brain regions. These findings suggest that Filamin-A may physically link metabotropic glutamate receptors to the actin cytoskeleton.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Animals
  • Brain / metabolism
  • Cattle
  • Cell Line
  • Contractile Proteins / genetics
  • Contractile Proteins / metabolism*
  • Filamins
  • Humans
  • Mice
  • Microfilament Proteins / genetics
  • Microfilament Proteins / metabolism*
  • Organ Specificity
  • Protein Binding / physiology
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • Protein Structure, Tertiary / physiology
  • Rats
  • Receptors, Metabotropic Glutamate / genetics
  • Receptors, Metabotropic Glutamate / metabolism*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Two-Hybrid System Techniques

Substances

  • Contractile Proteins
  • Filamins
  • Microfilament Proteins
  • Protein Isoforms
  • Receptors, Metabotropic Glutamate
  • Recombinant Proteins
  • metabotropic glutamate receptor 7