Abstract
A yeast two-hybrid screen identified Filamin-A as a binding partner of the metabotropic glutamate receptor type 7b (mGluR7b) splice variant. In addition, Filamin-A interacted with mGluR4a, mGluR5a, mGluR5b, mGluR7a and mGluR8a. Domain mapping revealed that alternative splicing of mGluR4, mGluR7 and mGluR8 C-termini regulated the interaction. A conserved tyrosine within mGluR C-termini was identified to mediate the binding to Filamin-A. Protein interactions were verified in biochemical assays using recombinant and native proteins. Finally, co-expression of Filamin-A and mGluR7 splice variants was shown in brain regions. These findings suggest that Filamin-A may physically link metabotropic glutamate receptors to the actin cytoskeleton.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Animals
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Brain / metabolism
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Cattle
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Cell Line
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Contractile Proteins / genetics
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Contractile Proteins / metabolism*
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Filamins
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Humans
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Mice
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Microfilament Proteins / genetics
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Microfilament Proteins / metabolism*
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Organ Specificity
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Protein Binding / physiology
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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Protein Structure, Tertiary / physiology
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Rats
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Receptors, Metabotropic Glutamate / genetics
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Receptors, Metabotropic Glutamate / metabolism*
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Two-Hybrid System Techniques
Substances
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Contractile Proteins
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Filamins
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Microfilament Proteins
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Protein Isoforms
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Receptors, Metabotropic Glutamate
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Recombinant Proteins
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metabotropic glutamate receptor 7