Cloning and tissue distribution of three murine alpha/beta hydrolase fold protein cDNAs

Biochem Biophys Res Commun. 2002 Apr 5;292(3):617-25. doi: 10.1006/bbrc.2002.6692.

Abstract

We have cloned 3 novel murine cDNAs encoding proteins containing an alpha/beta hydrolase fold; a catalytic domain found in a very wide range of enzymes. These proteins belong to the prosite UPF0017 uncharacterized protein family and we have named them lung alpha/beta hydrolase 1, 2, and 3 (LABH) since they were cloned from lung cDNA. All have 9 coding exons, encoding 412, 425, and 411 residue proteins respectively (46-48 kDa); LABH1 being closely related to LABH3 having 45% identity. All 3 proteins have a single predicted amino-terminus transmembrane domain. An alignment of family members from different phyla enabled the identification of the LABH1 catalytic triad as Ser211, Asp337, and His366. mRNA expression levels of LABH1 and 3 were highest in liver and LABH2 highest in testis. These findings suggest that the LABH proteins consist of a novel family of membrane bound enzymes whose function has yet to be determined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Catalytic Domain
  • DNA, Complementary
  • Emphysema / enzymology
  • Emphysema / genetics
  • Humans
  • Hydrolases / chemistry*
  • Hydrolases / classification
  • Hydrolases / genetics
  • Hydrolases / metabolism*
  • Liver / enzymology
  • Lung / enzymology
  • Male
  • Membrane Proteins
  • Mice
  • Molecular Sequence Data
  • Phospholipases A2
  • Phylogeny
  • Protein Folding
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Sequence Alignment
  • Testis / enzymology
  • Tissue Distribution

Substances

  • DNA, Complementary
  • Membrane Proteins
  • Hydrolases
  • LABH1, mouse
  • Abhd2 protein, mouse
  • Abhd3 protein, mouse
  • Phospholipases A2

Associated data

  • GENBANK/AF189764
  • GENBANK/AF201730
  • GENBANK/AF429302