Molecular cloning of ADIR, a novel interferon responsive gene encoding a protein related to the torsins

Genomics. 2002 Mar;79(3):315-25. doi: 10.1006/geno.2002.6709.

Abstract

The expression of the previously uncharacterized gene Adir (for ATP dependent interferon responsive gene) was increased by 5- to 15-fold in tissue of the oral cavity or in spleen and liver of mice treated orally or intraperitoneally with IFN-alpha, and in mouse cells treated in vitro with IFN-alpha or IFN-gamma. The level of Adir mRNA was also increased 20- to 40-fold in the brains of animals infected with encephalomyocarditis virus. Adir is expressed ubiquitously in mouse tissues as 1.9-, 2.4-, and 3.5-kb mRNA transcripts encoding a 385-amino-acid protein with a conserved ATP binding domain containing typical nucleotide and Mg(2+) binding sites. We also characterized the human ortholog, ADIR, which is located on chromosome 1q25-q31 and contains six exons encoding a 397-amino-acid protein with 80% homology to the mouse protein. A single 2.3-kb mRNA was detected in all human tissues examined, except for placenta, which also contained a 1.25-kb tissue-specific transcript generated by alternative splicing and encoding a putative 336-amino-acid protein. Although ADIR exhibits low homology to DYT1 and TOR1B, the deduced ADIR protein sequences are highly homologous to torsin A and torsin B and more distantly related to members of the Clp/HSP100 family of proteins, suggesting that ADIR, like torsins, is related to the AAA chaperone-like family of ATPases. An ADIR-EGFP fusion protein expressed in HeLa cells was shown to be associated with the endoplasmic reticulum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics*
  • Adenosine Triphosphate / metabolism
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins / genetics*
  • Cloning, Molecular
  • Humans
  • Interferons / genetics*
  • Interferons / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Minor Histocompatibility Antigens
  • Molecular Chaperones / genetics*
  • Molecular Sequence Data
  • Sequence Homology

Substances

  • Carrier Proteins
  • Dyt1 protein, mouse
  • Minor Histocompatibility Antigens
  • Molecular Chaperones
  • TOR1A protein, human
  • TOR1B protein, human
  • Tor1b protein, mouse
  • Adenosine Triphosphate
  • Interferons
  • Adenosine Triphosphatases
  • TOR3A protein, human
  • Tor3a protein, mouse

Associated data

  • GENBANK/AJ299403
  • GENBANK/AJ299404
  • GENBANK/AJ299441
  • GENBANK/AJ318043
  • GENBANK/AJ318044
  • GENBANK/AJ318045
  • GENBANK/AJ318046
  • GENBANK/AJ318047
  • GENBANK/AJ318048
  • GENBANK/AJ318049
  • GENBANK/AJ318050