Molecular characterization of the TCP11 gene which is the human homologue of the mouse gene encoding the receptor of fertilization promoting peptide

Yongxin Ma; Sizhong Zhang; Qinjie Xia; Ge Zhang; Xinli Huang; Mingkong Huang; Cuiying Xiao; Agen Pan; Yan Sun; Roger Lebo; Aubrey Milunsky

doi:10.1093/molehr/8.1.24

Molecular characterization of the TCP11 gene which is the human homologue of the mouse gene encoding the receptor of fertilization promoting peptide

Mol Hum Reprod. 2002 Jan;8(1):24-31. doi: 10.1093/molehr/8.1.24.

Authors

Yongxin Ma¹, Sizhong Zhang, Qinjie Xia, Ge Zhang, Xinli Huang, Mingkong Huang, Cuiying Xiao, Agen Pan, Yan Sun, Roger Lebo, Aubrey Milunsky

Affiliation

¹ Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu, 610041, China.

PMID: 11756566
DOI: 10.1093/molehr/8.1.24

Abstract

A human testis-specific gene was isolated by subtractive hybridization between the cDNA pools of adult and fetal testes, followed by rapid amplification of cDNA ends (RACE). This gene sequence is highly homologous to a large portion of the mouse Tcp11 gene which is important in sperm function because it encodes the receptor for fertilization-promoting peptide (FPP). The gene was mapped to human chromosome band 6p21 by fluorescence in-situ hybridization. The 9 exon gene spans a 22.8 kp genomic DNA sequence. The mature processed message encodes a 441 amino acid protein that is highly homologous to the mouse 566 amino acid protein after the first 142 amino acids. Results of Northern blot and RT-PCR analyses of RNA extracted from human tissues revealed that the gene is only expressed in fertile adult testes, but not in azoospermic testes, fetal testes nor in other human tissues. Taken together, our results along with the mouse Tcp11 function suggest that TCP11 gene is important in sperm function and fertility.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 6
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Exons / genetics
Humans
In Situ Hybridization, Fluorescence
Intracellular Signaling Peptides and Proteins*
Male
Membrane Proteins
Mice
Microtubule-Associated Proteins*
Molecular Sequence Data
Nuclear Proteins / chemistry
Nuclear Proteins / genetics*
Nuclear Proteins / metabolism*
Nucleic Acid Hybridization / methods
Pyrrolidonecarboxylic Acid / analogs & derivatives
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism
Sequence Alignment
Testicular Hormones / genetics
Testicular Hormones / metabolism
Testis / physiology*
Thyrotropin-Releasing Hormone / analogs & derivatives*
Thyrotropin-Releasing Hormone / metabolism
Ubiquitin-Protein Ligases
t-Complex Genome Region

Substances

DNA-Binding Proteins
Intracellular Signaling Peptides and Proteins
Membrane Proteins
Microtubule-Associated Proteins
Nuclear Proteins
Receptors, Cell Surface
TCP11 protein, human
Tcp11 protein, mouse
Testicular Hormones
Thyrotropin-Releasing Hormone
pyroglutamyl-glutamyl-proline amide
PPP1R11 protein, human
Ubiquitin-Protein Ligases
Pyrrolidonecarboxylic Acid