The paralemmin protein family: identification of paralemmin-2, an isoform differentially spliced to AKAP2/AKAP-KL, and of palmdelphin, a more distant cytosolic relative

Biochem Biophys Res Commun. 2001 Aug 3;285(5):1369-76. doi: 10.1006/bbrc.2001.5329.

Abstract

Paralemmin is a protein implicated in plasma membrane dynamics. Here we describe the identification of two new paralemmin-related proteins. A partial paralemmin homolog, palmdelphin, is predominantly cytosolic, unlike paralemmin which is lipid-anchored to the plasma membrane through a C-terminal CaaX motif. We have mapped the mouse palmdelphin gene to distal chromosome 3 between Amy2 and Abcd3, in a region homologous to human chromosome 1p22-p21 where the human palmdelphin gene is located. We have also identified a second paralemmin isoform, paralemmin-2. It is expressed from a gene on human chromosome 9q31-q33 which ends only 33 kb upstream of the gene encoding the protein kinase A-binding protein,AKAP2/AKAP-KL. The closely adjacent paralemmin-2 and AKAP2 genes are functionally linked in a very unusual manner. Chimeric mRNAs are expressed, apparently by RNA readthrough and differential splicing, that encode natural fusion proteins in which either the N-terminal coiled-coil region or nearly the complete sequence of paralemmin-2 except its C-terminal CaaX motif is fused to AKAP2/AKAP-KL. The N-terminal coiled-coil region is conserved in paralemmin-1, paralemmin-2/AKAP2, palmdelphin and a fourth, uncharacterized gene, suggesting that it is a modular functional domain.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • A Kinase Anchor Proteins
  • Adaptor Proteins, Signal Transducing*
  • Alternative Splicing
  • Animals
  • Carrier Proteins / genetics*
  • Chickens
  • Chromosomes, Human, Pair 1 / genetics
  • Chromosomes, Human, Pair 9 / genetics
  • Cytosol / metabolism
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Humans
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Mice
  • Molecular Sequence Data
  • Multigene Family*
  • Organ Specificity
  • Phosphoproteins
  • Physical Chromosome Mapping
  • Protein Biosynthesis
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • Protein Structure, Tertiary
  • Proteins / genetics*
  • Proteins / metabolism
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Rats
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Terminology as Topic

Substances

  • A Kinase Anchor Proteins
  • AKAP2 protein, human
  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • DNA, Complementary
  • Membrane Proteins
  • PALM protein, human
  • Pakap protein, mouse
  • Palm protein, mouse
  • Palm2-AKAP2 fusion protein, human
  • Palmd protein, mouse
  • Phosphoproteins
  • Protein Isoforms
  • Proteins
  • RNA, Messenger
  • Recombinant Fusion Proteins

Associated data

  • GENBANK/AJ312214
  • GENBANK/AJ312215
  • GENBANK/AJ312216
  • GENBANK/AJ312217