Effect of selenium and vitamin E deficiency on differential gene expression in rat liver

Biochem Biophys Res Commun. 2001 Jul 13;285(2):470-5. doi: 10.1006/bbrc.2001.5171.

Abstract

To examine the molecular events associated with selenium (Se) and vitamin E (VE) deficiency, we applied cDNA array technology to define the transcriptional response in the liver of Se- and VE-deficient rats. VE deficiency alone did not induce any significant changes in expression profile among the genes evaluated. Se deficiency lead to a down-regulation of Se-dependent cGPx and to an induction of genes, encoding for detoxifying enzymes in liver (cytochrome P450 4B1, UDP-glucuronosyltransferase 1). Combined VE and Se deficiency was characterized by alterations in the expression level of genes encoding for proteins involved in inflammation (multispecific organic anion exporter, SPI-3 serine protease inhibitor) and acute phase response (alpha-1 acid glycoprotein, metallothionein 1). Additionally, a significant down-regulation in the expression level of genes important in the inhibition of apoptosis (defender against cell death 1 protein, Bcl2-L1), cell cycle (G1/S-specific cyclin D1) and antioxidant defense (gamma-glutamylcysteine synthetase catalytic subunit) was demonstrated. The experimental strategy identified several novel Se and VE sensitive genes.

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Apoptosis / physiology
  • Aryl Hydrocarbon Hydroxylases*
  • Cell Cycle / drug effects
  • Cell Cycle / physiology
  • Cytochrome P-450 Enzyme System / biosynthesis
  • Cytochrome P-450 Enzyme System / genetics
  • Dihydrolipoamide Dehydrogenase / biosynthesis
  • Dihydrolipoamide Dehydrogenase / genetics
  • Enzyme Induction
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology*
  • Glucuronosyltransferase / biosynthesis
  • Glucuronosyltransferase / genetics
  • Glutathione / metabolism
  • Glutathione Peroxidase / biosynthesis
  • Glutathione Peroxidase / genetics*
  • Liver / cytology
  • Liver / drug effects
  • Liver / physiology*
  • Metallothionein / metabolism
  • Rats
  • Selenium / deficiency*
  • Selenium / pharmacology
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Tissue Inhibitor of Metalloproteinase-1 / genetics
  • Vitamin E / pharmacology*
  • Vitamin E Deficiency / metabolism*

Substances

  • Thiobarbituric Acid Reactive Substances
  • Tissue Inhibitor of Metalloproteinase-1
  • Vitamin E
  • Cytochrome P-450 Enzyme System
  • Metallothionein
  • Glutathione Peroxidase
  • Aryl Hydrocarbon Hydroxylases
  • cytochrome P-450 CYP4B1
  • Dihydrolipoamide Dehydrogenase
  • Glucuronosyltransferase
  • Glutathione
  • Selenium