Abstract
Ke 6 is a 17beta-hydroxysteroid dehydrogenase (17betaHSD) that is expressed in the kidneys and gonads. The expression of this gene is markedly reduced in three murine models of recessive polycystic kidney disease, a developmental disorder, where some nephrons within the affected kidneys develop into huge fluid-filled cysts while the non-cystic nephrons atrophies by apoptosis. Here, we show that in the cpk/cpk mouse, which have polycystic kidneys, the female reproductive organs also fail to mature properly and remain arrested at an early stage of development. Direct measurement of 17betaHSD activity showed a severe reduction in estrogen and androgen metabolism within gonadal and non-gonadal tissues of the cpk/cpk mouse. Using immunofluorescent staining we localized the expression of the Ke 6 protein within the female mouse reproductive organs. Our findings suggest that estrogen/androgen metabolism may play an important role in the development of the urogenital systems.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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17-Hydroxysteroid Dehydrogenases / metabolism
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Animals
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Chorionic Gonadotropin / pharmacology
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Dihydrotestosterone / metabolism
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Disease Models, Animal*
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Estradiol / metabolism
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Estrone / metabolism
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Fallopian Tubes / metabolism
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Female
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Histocompatibility Antigens / metabolism
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Homozygote
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Immunohistochemistry
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Kidney / enzymology
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Kidney / metabolism
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Liver / enzymology
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Liver / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Mutant Strains
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Mutation / genetics
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Ovary / drug effects
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Ovary / enzymology
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Ovary / metabolism*
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Ovary / pathology*
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Oxidoreductases*
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Polycystic Kidney, Autosomal Recessive / enzymology
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Polycystic Kidney, Autosomal Recessive / genetics
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Polycystic Kidney, Autosomal Recessive / metabolism*
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Polycystic Kidney, Autosomal Recessive / pathology*
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Rats
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Steroids / metabolism*
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Superovulation / drug effects
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Testosterone / metabolism
Substances
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Chorionic Gonadotropin
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H2-Ke6 protein, mouse
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Histocompatibility Antigens
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Steroids
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Dihydrotestosterone
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Estrone
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Testosterone
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Estradiol
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Oxidoreductases
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17-Hydroxysteroid Dehydrogenases
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3 (or 17)-beta-hydroxysteroid dehydrogenase