Abstract
The mammalian mitochondrial ribosome (mitoribosome) is a highly protein-rich particle in which almost half of the rRNA contained in the bacterial ribosome is replaced with proteins. It is known that mitochondrial translation factors can function on both mitochondrial and Escherichia coli ribosomes, indicating that protein components in the mitoribosome compensate the reduced rRNA chain to make a bacteria-type ribosome. To elucidate the molecular basis of this compensation, we analyzed bovine mitoribosomal large subunit proteins; 31 proteins were identified including 15 newly identified proteins with their cDNA sequences from human and mouse. The results showed that the proteins with binding sites on rRNA shortened or lost in the mitoribosome were enlarged when compared with the E. coli counterparts; this suggests the structural compensation of the rRNA deficit by the enlarged proteins in the mitoribosome.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Cattle
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DNA, Complementary
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Escherichia coli / genetics
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Humans
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Liver / chemistry
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Liver / physiology*
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Mice
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Mitochondria, Liver / physiology*
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Mitochondria, Liver / ultrastructure
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Models, Molecular
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Molecular Sequence Data
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Nucleic Acid Conformation
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Peptide Fragments / chemistry
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RNA, Ribosomal / chemistry*
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RNA, Ribosomal / genetics
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Ribosomal Proteins / chemistry*
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Ribosomal Proteins / genetics
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Ribosomal Proteins / isolation & purification
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Ribosomes / chemistry*
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Ribosomes / genetics
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Ribosomes / ultrastructure
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Sequence Alignment
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Sequence Homology, Amino Acid
Substances
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DNA, Complementary
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Peptide Fragments
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RNA, Ribosomal
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Ribosomal Proteins
Associated data
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GENBANK/AB049632
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GENBANK/AB049633
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GENBANK/AB049634
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GENBANK/AB049635
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GENBANK/AB049636
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GENBANK/AB049637
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GENBANK/AB049638
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GENBANK/AB049639
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GENBANK/AB049640
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GENBANK/AB049641
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GENBANK/AB049642
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GENBANK/AB049643
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GENBANK/AB049644
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GENBANK/AB049645
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GENBANK/AB049646
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GENBANK/AB049647
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GENBANK/AB049648
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GENBANK/AB049649
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GENBANK/AB049650
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GENBANK/AB049651
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GENBANK/AB049652
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GENBANK/AB049653
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GENBANK/AB049654
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GENBANK/AB049655
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GENBANK/AB049656
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GENBANK/AB049657