Differential gene expression of beta-1,4-galactosyltransferases I, II and V during mouse brain development

J Neurochem. 2001 Jan;76(1):29-38. doi: 10.1046/j.1471-4159.2001.00004.x.

Abstract

Since most brain glycoproteins from beta-1,4-galactosyltransferase (beta-1,4-GalT) I knockout mice were galactosylated without apparent reduction the gene expression of novel beta-1,4-GalTs II and V which are involved in N-linked oligosaccharide biosynthesis in addition to beta-1,4-GalT I was studied during mouse brain development. Isolation and characterization of beta-1,4-GalT II and V cDNAs from mouse brains indicates that they are also functioning in the brain. Northern blot analysis revealed that the beta-1,4-GalT I gene is expressed mainly in mid-embryonic stages, while the expression level of beta-1,4-GalT II transcript remains constant and of beta-1,4-GalT V transcript increases during mouse brain development after birth. In situ hybridization revealed that beta-1,4-GalT II and V signals are present in most neural cells, with a marked difference between them in the hippocampus of adult mouse brain tissue. The differential gene expression of beta-1,4-GalTs I, II and V during mouse brain development could affect the differential galactosylation of brain glycoproteins, as revealed by lectin blot analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / metabolism
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Western
  • Brain / embryology
  • Brain / enzymology*
  • Brain / growth & development
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Galactosyltransferases / biosynthesis*
  • Galactosyltransferases / genetics
  • Gene Expression Regulation, Developmental*
  • In Situ Hybridization
  • Isoenzymes / biosynthesis
  • Isoenzymes / genetics
  • Lectins
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Organ Specificity
  • RNA, Messenger / biosynthesis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • Isoenzymes
  • Lectins
  • RNA, Messenger
  • Galactosyltransferases
  • beta-1,4-galactosyltransferase I
  • beta-1,4-galactosyltransferase II
  • beta-1,4-galactosyltransferase V

Associated data

  • GENBANK/AB004786
  • GENBANK/AB019541