The establishment of a human liver nuclei two-dimensional electrophoresis reference map

Electrophoresis. 2000 Oct;21(16):3483-7. doi: 10.1002/1522-2683(20001001)21:16<3483::AID-ELPS3483>3.0.CO;2-X.

Abstract

This short communication describes the establishment of a two-dimensional electrophoresis (2-DE) reference map of nuclear proteins isolated from human liver. The human liver nuclei 2-DE reference map contains 1497 spots. In an initial identification study using peptide mass fingerprinting as a means of protein identification we were able to identify 26 spots corresponding to 15 different proteins. The human liver nuclei 2-DE reference map is now included in the SWISS-2DPAGE database, which can be accessed through the ExPASy server (http://www.expasy.ch/ch2d/).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / metabolism
  • Electrophoresis, Gel, Two-Dimensional
  • Humans
  • Liver / metabolism*
  • Liver / ultrastructure
  • Nuclear Proteins / isolation & purification
  • Nuclear Proteins / metabolism

Substances

  • Nuclear Proteins