cDNA cloning and tissue-specific splicing variants of mouse hippostasin/TLSP (PRSS20)

Biochim Biophys Acta. 2000 Nov 15;1494(1-2):206-10. doi: 10.1016/s0167-4781(00)00206-2.

Abstract

Two splicing variants of mouse hippostasin/TLSP (PRSS20) were identified and termed brain-type and prostate-type, respectively. Mouse hippostasin/TLSP showed 76.8% identity to the human homologue. Transient expression showed that both translational products were secreted into the conditioned medium. Mouse hippostasin/TLSP was expressed preferentially in the fetal brain and the prostate, but not in the neonatal brain. The brain expressed only brain-type hippostasin/TLSP, while the prostate expressed both types.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing / genetics*
  • Amino Acid Sequence
  • Animals
  • Animals, Newborn
  • Base Sequence
  • Brain / embryology
  • Brain / enzymology
  • Brain / metabolism*
  • COS Cells
  • Cloning, Molecular
  • Culture Media, Conditioned / metabolism
  • DNA, Complementary / genetics
  • Expressed Sequence Tags
  • Humans
  • Male
  • Mice
  • Molecular Sequence Data
  • Organ Specificity
  • Prostate / embryology
  • Prostate / enzymology
  • Prostate / metabolism*
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Sequence Alignment
  • Sequence Homology
  • Serine Endopeptidases / genetics*
  • Serine Endopeptidases / metabolism
  • Transfection

Substances

  • Culture Media, Conditioned
  • DNA, Complementary
  • RNA, Messenger
  • trypsin-like serine protease
  • Serine Endopeptidases

Associated data

  • GENBANK/AB016226
  • GENBANK/AB016227