Purification, crystallization and preliminary X-ray crystallographic analysis of human CCG1-interacting factor B

B Padmanabhan; T Kuzuhara; H Mizuno; M Horikoshi

doi:10.1107/s0907444900010957

Purification, crystallization and preliminary X-ray crystallographic analysis of human CCG1-interacting factor B

Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1479-81. doi: 10.1107/s0907444900010957.

Authors

B Padmanabhan¹, T Kuzuhara, H Mizuno, M Horikoshi

Affiliation

¹ Horikoshi Gene Selector Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Corporation (JST), 5-9-6 Tokodai, Tsukuba, Ibaraki 300-2635, Japan.

PMID: 11053859
DOI: 10.1107/s0907444900010957

Abstract

A novel human factor CIB (CCG1-interacting factor B) has been isolated using the yeast two-hybrid system. The 22 kDa CIB protein has been expressed in Escherichia coli, purified to homogeneity and crystallized in a form suitable for crystallographic studies. The protein was crystallized in the orthogonal space group P2(1)2(1)2(1), with unit-cell parameters a = 43.60 (2), b = 44.45 (1), c = 110.70 (5) A and one molecule in the asymmetric unit. The crystal diffracted beyond 2.2 A resolution using synchrotron radiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Cycle Proteins / chemistry*
Crystallography
Crystallography, X-Ray
Humans
Open Reading Frames
Protein Conformation

Substances

Cell Cycle Proteins