Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm

Mol Cell. 2000 Sep;6(3):673-82. doi: 10.1016/s1097-2765(00)00065-4.

Abstract

We describe a novel RNA binding protein, Y14, a predominantly nuclear nucleocytoplasmic shuttling protein. Interestingly, Y14 associates preferentially with mRNAs produced by splicing but not with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Y14 associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. Splicing of a single intron is sufficient for Y14 association. Y14-containing nuclear complexes are different from general hnRNP complexes. They contain hnRNP proteins and several unique proteins including the mRNA export factor TAP. Thus, Y14 defines novel intermediates in the pathway of gene expression, postsplicing nuclear preexport mRNPs, and newly exported cytoplasmic mRNPs, whose composition is established by splicing. These findings suggest that pre-mRNA splicing imprints mRNA with a unique set of proteins that persists in the cytoplasm and thereby communicates the history of the transcript.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Nucleus / chemistry
  • Cell Nucleus / genetics
  • Cell Nucleus / metabolism
  • Cytoplasm / chemistry
  • Cytoplasm / genetics
  • Cytoplasm / metabolism
  • HeLa Cells
  • Heterogeneous-Nuclear Ribonucleoproteins
  • Humans
  • Introns / genetics
  • Molecular Sequence Data
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Oocytes / physiology
  • RNA Precursors / genetics*
  • RNA Precursors / metabolism
  • RNA Splicing / physiology*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*
  • RNA-Binding Proteins / analysis
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / metabolism*
  • Ribonucleoproteins / genetics
  • Ribonucleoproteins / metabolism
  • Sequence Homology, Amino Acid
  • Xenopus
  • beta Karyopherins*
  • ran GTP-Binding Protein / genetics
  • ran GTP-Binding Protein / metabolism

Substances

  • Heterogeneous-Nuclear Ribonucleoproteins
  • IPO5 protein, human
  • Nuclear Proteins
  • RBM8A protein, human
  • RNA Precursors
  • RNA, Messenger
  • RNA-Binding Proteins
  • Ribonucleoproteins
  • beta Karyopherins
  • ran GTP-Binding Protein

Associated data

  • GENBANK/AF299118
  • GENBANK/AF299119