Abstract
This report describes a new human B7-like gene designated B7-H2. Cell surface expression of B7-H2 protein is detected in monocyte-derived immature dendritic cells. Soluble B7-H2 and immunoglobulin (Ig) fusion protein, B7-H2Ig, binds activated but not resting T cells and the binding is abrogated by inducible costimulator Ig (ICOSIg), but not CTLA4Ig. In addition, ICOSIg stains Chinese hamster ovary cells transfected with B7-H2 gene. By suboptimal cross-linking of CD3, costimulation of T-cell proliferation by B7-H2Ig is dose-dependent and correlates with secretion of interleukin (IL)-2, whereas optimal CD3 ligation preferentially stimulates IL-10 production. The results indicate that B7-H2 is a putative ligand for the ICOS T-cell molecule. (Blood. 2000;96:2808-2813)
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Abatacept
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Amino Acid Sequence
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Animals
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Antigens, CD
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Antigens, Differentiation / immunology
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Antigens, Differentiation, T-Lymphocyte / metabolism*
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CD3 Complex / immunology
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CHO Cells
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CTLA-4 Antigen
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Carrier Proteins / chemistry
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Carrier Proteins / genetics
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Carrier Proteins / physiology*
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Cell Division
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Cloning, Molecular
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Cricetinae
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Cricetulus
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DNA, Complementary / genetics
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Dendritic Cells / immunology
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Dendritic Cells / metabolism
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Genes, Immunoglobulin
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Humans
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Immunoconjugates*
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Immunoglobulin G / genetics
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Inducible T-Cell Co-Stimulator Ligand
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Inducible T-Cell Co-Stimulator Protein
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Interleukin-10 / metabolism
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Interleukin-2 / metabolism
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Ligands
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Lymphocyte Activation / physiology*
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Protein Structure, Tertiary
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Proteins / chemistry
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Recombinant Fusion Proteins / physiology
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Sequence Alignment
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Sequence Homology, Amino Acid
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T-Lymphocytes / immunology*
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T-Lymphocytes / metabolism
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Transfection
Substances
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Antigens, CD
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Antigens, Differentiation
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Antigens, Differentiation, T-Lymphocyte
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CD3 Complex
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CTLA-4 Antigen
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CTLA4 protein, human
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Carrier Proteins
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Ctla4 protein, mouse
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DNA, Complementary
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ICOS protein, human
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ICOSLG protein, human
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Icos protein, mouse
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Icosl protein, mouse
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Immunoconjugates
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Immunoglobulin G
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Inducible T-Cell Co-Stimulator Ligand
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Inducible T-Cell Co-Stimulator Protein
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Interleukin-2
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Ligands
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Proteins
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Recombinant Fusion Proteins
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Interleukin-10
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Abatacept