Costimulation of T cells by B7-H2, a B7-like molecule that binds ICOS

Blood. 2000 Oct 15;96(8):2808-13.

Abstract

This report describes a new human B7-like gene designated B7-H2. Cell surface expression of B7-H2 protein is detected in monocyte-derived immature dendritic cells. Soluble B7-H2 and immunoglobulin (Ig) fusion protein, B7-H2Ig, binds activated but not resting T cells and the binding is abrogated by inducible costimulator Ig (ICOSIg), but not CTLA4Ig. In addition, ICOSIg stains Chinese hamster ovary cells transfected with B7-H2 gene. By suboptimal cross-linking of CD3, costimulation of T-cell proliferation by B7-H2Ig is dose-dependent and correlates with secretion of interleukin (IL)-2, whereas optimal CD3 ligation preferentially stimulates IL-10 production. The results indicate that B7-H2 is a putative ligand for the ICOS T-cell molecule. (Blood. 2000;96:2808-2813)

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Abatacept
  • Amino Acid Sequence
  • Animals
  • Antigens, CD
  • Antigens, Differentiation / immunology
  • Antigens, Differentiation, T-Lymphocyte / metabolism*
  • CD3 Complex / immunology
  • CHO Cells
  • CTLA-4 Antigen
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics
  • Carrier Proteins / physiology*
  • Cell Division
  • Cloning, Molecular
  • Cricetinae
  • Cricetulus
  • DNA, Complementary / genetics
  • Dendritic Cells / immunology
  • Dendritic Cells / metabolism
  • Genes, Immunoglobulin
  • Humans
  • Immunoconjugates*
  • Immunoglobulin G / genetics
  • Inducible T-Cell Co-Stimulator Ligand
  • Inducible T-Cell Co-Stimulator Protein
  • Interleukin-10 / metabolism
  • Interleukin-2 / metabolism
  • Ligands
  • Lymphocyte Activation / physiology*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • Proteins / chemistry
  • Recombinant Fusion Proteins / physiology
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism
  • Transfection

Substances

  • Antigens, CD
  • Antigens, Differentiation
  • Antigens, Differentiation, T-Lymphocyte
  • CD3 Complex
  • CTLA-4 Antigen
  • CTLA4 protein, human
  • Carrier Proteins
  • Ctla4 protein, mouse
  • DNA, Complementary
  • ICOS protein, human
  • ICOSLG protein, human
  • Icos protein, mouse
  • Icosl protein, mouse
  • Immunoconjugates
  • Immunoglobulin G
  • Inducible T-Cell Co-Stimulator Ligand
  • Inducible T-Cell Co-Stimulator Protein
  • Interleukin-2
  • Ligands
  • Proteins
  • Recombinant Fusion Proteins
  • Interleukin-10
  • Abatacept