Cloning, chromosome mapping and functional characterization of a human homologue of murine gtse-1 (B99) gene

Gene. 2000 Aug 22;254(1-2):229-36. doi: 10.1016/s0378-1119(00)00260-2.

Abstract

Murine Gtse-1 (G(2) and S phase expressed protein), previously named B99, is a wt-p53 inducible gene that encodes a microtubule-localized protein which is able to induce G(2)/M phase accumulation when ectopically expressed. Here we report the cloning and characterization of a new cDNA (GTSE-1) encoding a human homologue of the mouse Gtse-1 protein. Chromosome mapping of mouse and human genes assigned Gtse-1 to chromosome 15 and GTSE-1 to chromosome 22q13.2-q13.3 in a region with conserved synteny to that where Gtse-1 mapped. Analysis of the genomic structure revealed that GTSE-1 contains at least 11 exons and 10 introns, spanning approximately 33kb of genomic DNA. Similar to murine Gtse-1, the product of GTSE-1 localized to the microtubules, was able to delay G(2)/M progression when ectopically expressed and was cell cycle regulated. Taken together, these results indicate GTSE-1 as the human functional homologue of murine Gtse-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Western
  • COS Cells
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism
  • Cell Line
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Exons
  • Gene Expression
  • Genes / genetics
  • Humans
  • Introns
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Microscopy, Fluorescence
  • Microtubule-Associated Proteins / genetics*
  • Microtubule-Associated Proteins / metabolism
  • Molecular Sequence Data
  • Muridae
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Radiation Hybrid Mapping
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tumor Cells, Cultured

Substances

  • Cell Cycle Proteins
  • DNA, Complementary
  • GTSE1 protein, human
  • Gtse1 protein, mouse
  • Microtubule-Associated Proteins
  • RNA, Messenger

Associated data

  • GENBANK/AF223408