Abstract
Use of an NF-kappaB-dependent selectable marker facilitated the isolation of a cell line containing a cDNA encoding Act1, an NF-kappaB activator. Act1 associates with and activates IkappaB kinase (IKK), leading to the liberation of NF-kappaB from its complex with IkappaB. Many signaling pathways that liberate NF-kappaB also activate activating transcription factor (ATF) and activator protein 1 (AP-1) through Jun kinase (JNK). Act1 also activates JNK, suggesting that it might be part of a multifunctional complex involved in the activation of both NF-kappaB and JNK. Act1 fails to activate NF-kappaB in an IL-1-unresponsive mutant cell line in which all known signaling components are present, suggesting that it interacts with an unknown component in IL-1 signaling.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adaptor Proteins, Signal Transducing*
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Amino Acid Sequence
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Carrier Proteins*
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Cell Line
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DNA, Complementary
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Enzyme Activation
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Helix-Loop-Helix Motifs
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Humans
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Interleukin-1 / metabolism
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JNK Mitogen-Activated Protein Kinases
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Mitogen-Activated Protein Kinases / metabolism
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Molecular Sequence Data
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NF-kappa B / metabolism*
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Recombinant Proteins / metabolism
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Sequence Homology, Nucleic Acid
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Trans-Activators / chemistry
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Trans-Activators / genetics*
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Trans-Activators / metabolism
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Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
Substances
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Adaptor Proteins, Signal Transducing
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Carrier Proteins
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DNA, Complementary
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Interleukin-1
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NF-kappa B
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Recombinant Proteins
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TRAF3IP2 protein, human
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Trans-Activators
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Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
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JNK Mitogen-Activated Protein Kinases
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Mitogen-Activated Protein Kinases