- Comment in:
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Nature. 2000 Aug 24;406(6798):826.
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Nature. 2000 Jul 13;406(6792):140-1.
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Nature. 2000 Nov 2;408(6808):17.
The genome sequence of the plant pathogen Xylella fastidiosa. The Xylella fastidiosa Consortium of the Organization for Nucleotide Sequencing and Analysis.
Simpson AJ,
Reinach FC,
Arruda P,
Abreu FA,
Acencio M,
Alvarenga R,
Alves LM,
Araya JE,
Baia GS,
Baptista CS,
Barros MH,
Bonaccorsi ED,
Bordin S,
Bové JM,
Briones MR,
Bueno MR,
Camargo AA,
Camargo LE,
Carraro DM,
Carrer H,
Colauto NB,
Colombo C,
Costa FF,
Costa MC,
Costa-Neto CM,
Coutinho LL,
Cristofani M,
Dias-Neto E,
Docena C,
El-Dorry H,
Facincani AP,
Ferreira AJ,
Ferreira VC,
Ferro JA,
Fraga JS,
França SC,
Franco MC,
Frohme M,
Furlan LR,
Garnier M,
Goldman GH,
Goldman MH,
Gomes SL,
Gruber A,
Ho PL,
Hoheisel JD,
Junqueira ML,
Kemper EL,
Kitajima JP,
Krieger JE,
Kuramae EE,
Laigret F,
Lambais MR,
Leite LC,
Lemos EG,
Lemos MV,
Lopes SA,
Lopes CR,
Machado JA,
Machado MA,
Madeira AM,
Madeira HM,
Marino CL,
Marques MV,
Martins EA,
Martins EM,
Matsukuma AY,
Menck CF,
Miracca EC,
Miyaki CY,
Monteriro-Vitorello CB,
Moon DH,
Nagai MA,
Nascimento AL,
Netto LE,
Nhani A Jr,
Nobrega FG,
Nunes LR,
Oliveira MA,
de Oliveira MC,
de Oliveira RC,
Palmieri DA,
Paris A,
Peixoto BR,
Pereira GA,
Pereira HA Jr,
Pesquero JB,
Quaggio RB,
Roberto PG,
Rodrigues V,
de M Rosa AJ,
de Rosa VE Jr,
de Sá RG,
Santelli RV,
Sawasaki HE,
da Silva AC,
da Silva AM,
da Silva FR,
da Silva WA Jr,
da Silveira JF,
Silvestri ML,
Siqueira WJ,
de Souza AA,
de Souza AP,
Terenzi MF,
Truffi D,
Tsai SM,
Tsuhako MH,
Vallada H,
Van Sluys MA,
Verjovski-Almeida S,
Vettore AL,
Zago MA,
Zatz M,
Meidanis J,
Setubal JC.
Instituto Ludwig de Pesquisa sobre o Câncer, São Paulo, SP, Brazil.
Xylella fastidiosa is a fastidious, xylem-limited bacterium that causes a range of economically important plant diseases. Here we report the complete genome sequence of X. fastidiosa clone 9a5c, which causes citrus variegated chlorosis--a serious disease of orange trees. The genome comprises a 52.7% GC-rich 2,679,305-base-pair (bp) circular chromosome and two plasmids of 51,158 bp and 1,285 bp. We can assign putative functions to 47% of the 2,904 predicted coding regions. Efficient metabolic functions are predicted, with sugars as the principal energy and carbon source, supporting existence in the nutrient-poor xylem sap. The mechanisms associated with pathogenicity and virulence involve toxins, antibiotics and ion sequestration systems, as well as bacterium-bacterium and bacterium-host interactions mediated by a range of proteins. Orthologues of some of these proteins have only been identified in animal and human pathogens; their presence in X. fastidiosa indicates that the molecular basis for bacterial pathogenicity is both conserved and independent of host. At least 83 genes are bacteriophage-derived and include virulence-associated genes from other bacteria, providing direct evidence of phage-mediated horizontal gene transfer.
PMID: 10910347 [PubMed - indexed for MEDLINE]