Abstract
IL-2 is the major autocrine and paracrine growth factor produced by T cells upon T cell stimulation. The inducible expression of IL-2 is highly regulated by multiple transcription factors, particularly AP-1, which coordinately activate the promoter. Described here is the ability of the novel basic leucine zipper protein p21SNFT to repress AP-1 activity and IL-2 transcription. A detailed analysis of the repression by p21SNFT repression on the IL-2 promoter distal NF-AT/AP-1 site demonstrates that it can bind DNA with NF-AT and Jun, strongly suggesting that it represses NF-AT/AP-1 activity by competing with Fos proteins for Jun dimerization. The importance of this repression is that p21SNFT inhibits the trans-activation potential of protein complexes that contain Jun, thereby demonstrating an additional level of control for the highly regulated, ubiquitous AP-1 transcription factor and the IL-2 gene.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Basic-Leucine Zipper Transcription Factors
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Binding Sites / genetics
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Binding Sites / immunology
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DNA-Binding Proteins / metabolism
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Down-Regulation / immunology
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Gene Expression Regulation / immunology
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Humans
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Interleukin-2 / antagonists & inhibitors*
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Interleukin-2 / genetics*
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Jurkat Cells / immunology
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Jurkat Cells / metabolism
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Leucine Zippers / genetics
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Leucine Zippers / immunology*
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Lymphocyte Activation / genetics
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Macromolecular Substances
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Molecular Sequence Data
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Molecular Weight
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NFATC Transcription Factors
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Nuclear Proteins*
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Promoter Regions, Genetic / immunology*
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Proto-Oncogene Proteins c-jun / metabolism
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Repressor Proteins / biosynthesis
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Repressor Proteins / metabolism
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Repressor Proteins / physiology*
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Transcription Factor AP-1 / metabolism
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Transcription Factors / biosynthesis
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Transcription Factors / metabolism
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Transcription Factors / physiology*
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Transfection / immunology
Substances
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BATF3 protein, human
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Basic-Leucine Zipper Transcription Factors
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DNA-Binding Proteins
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Interleukin-2
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Macromolecular Substances
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NFATC Transcription Factors
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Nuclear Proteins
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Proto-Oncogene Proteins c-jun
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Repressor Proteins
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Transcription Factor AP-1
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Transcription Factors