YAC/BAC-based physical and transcript mapping around the gracile axonal dystrophy (gad) locus identifies Uchl1, Pmx2b, Atp3a2, and Hip2 genes

Genomics. 2000 Jun 15;66(3):333-6. doi: 10.1006/geno.2000.6221.

Abstract

We generated a yeast artificial chromosome (YAC)/bacterial artificial chromosome (BAC)-based physical and transcript map of a region containing the gracile axonal dystrophy (gad) locus on mouse chromosome 5. The YAC/BAC contig consists of 13 YAC and 49 BAC clones onto which 4 genes, 40 expressed sequence tags, and 7 new DNA polymorphisms were ordered. Using this physical map, we mapped Uchl1 encoding ubiquitin carboxyl-terminal hydrolase I, whose deletion has been determined to cause the gad mutation. We also mapped three other recently identified genes: Hip2, encoding Huntingtin interacting protein 2; Atp3a2, encoding a P-type ATPase; and Pmx2b, encoding PHOX2b.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics
  • Animals
  • Carrier Proteins / genetics
  • Chromosome Walking
  • Chromosomes, Artificial, Yeast / genetics*
  • Chromosomes, Bacterial / genetics
  • Contig Mapping*
  • Exons / genetics
  • Expressed Sequence Tags
  • Genetic Markers
  • Homeodomain Proteins / genetics
  • Humans
  • Ligases / genetics
  • Membrane Proteins / genetics
  • Mice
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Neuroaxonal Dystrophies / genetics*
  • Phospholipid Transfer Proteins*
  • Physical Chromosome Mapping*
  • Thiolester Hydrolases / genetics
  • Transcription Factors*
  • Ubiquitin Thiolesterase
  • Ubiquitin-Conjugating Enzymes*

Substances

  • Carrier Proteins
  • Genetic Markers
  • Homeodomain Proteins
  • Membrane Proteins
  • NBPhox protein
  • Phospholipid Transfer Proteins
  • Transcription Factors
  • UBE2K protein, human
  • Ubiquitin-Conjugating Enzymes
  • Thiolester Hydrolases
  • Ubiquitin Thiolesterase
  • Adenosine Triphosphatases
  • Ligases

Associated data

  • GENBANK/AB036455
  • GENBANK/AB036456
  • GENBANK/AB036457
  • GENBANK/AB036458
  • GENBANK/AB036459
  • GENBANK/AB036460
  • GENBANK/AB036461
  • GENBANK/AB036463
  • GENBANK/AB036464
  • GENBANK/AB036465
  • GENBANK/AB036466
  • GENBANK/AB036467
  • GENBANK/AB036468
  • GENBANK/AB036469
  • GENBANK/AB036470
  • GENBANK/AB036471
  • GENBANK/AB036472
  • GENBANK/AB036473
  • GENBANK/AB036474
  • GENBANK/AB036475
  • GENBANK/AB036476
  • GENBANK/AB036477
  • GENBANK/AB036478
  • GENBANK/AB036479
  • GENBANK/AB036480
  • GENBANK/AB036481
  • GENBANK/AB036482
  • GENBANK/AB036483
  • GENBANK/AB036484