Abstract
We generated a yeast artificial chromosome (YAC)/bacterial artificial chromosome (BAC)-based physical and transcript map of a region containing the gracile axonal dystrophy (gad) locus on mouse chromosome 5. The YAC/BAC contig consists of 13 YAC and 49 BAC clones onto which 4 genes, 40 expressed sequence tags, and 7 new DNA polymorphisms were ordered. Using this physical map, we mapped Uchl1 encoding ubiquitin carboxyl-terminal hydrolase I, whose deletion has been determined to cause the gad mutation. We also mapped three other recently identified genes: Hip2, encoding Huntingtin interacting protein 2; Atp3a2, encoding a P-type ATPase; and Pmx2b, encoding PHOX2b.
Copyright 2000 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / genetics
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Animals
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Carrier Proteins / genetics
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Chromosome Walking
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Chromosomes, Artificial, Yeast / genetics*
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Chromosomes, Bacterial / genetics
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Contig Mapping*
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Exons / genetics
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Expressed Sequence Tags
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Genetic Markers
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Homeodomain Proteins / genetics
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Humans
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Ligases / genetics
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Membrane Proteins / genetics
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Mice
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Mice, Inbred Strains
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Molecular Sequence Data
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Neuroaxonal Dystrophies / genetics*
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Phospholipid Transfer Proteins*
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Physical Chromosome Mapping*
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Thiolester Hydrolases / genetics
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Transcription Factors*
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Ubiquitin Thiolesterase
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Ubiquitin-Conjugating Enzymes*
Substances
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Carrier Proteins
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Genetic Markers
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Homeodomain Proteins
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Membrane Proteins
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NBPhox protein
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Phospholipid Transfer Proteins
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Transcription Factors
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UBE2K protein, human
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Ubiquitin-Conjugating Enzymes
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Thiolester Hydrolases
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Ubiquitin Thiolesterase
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Adenosine Triphosphatases
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Ligases
Associated data
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GENBANK/AB036455
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GENBANK/AB036456
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GENBANK/AB036457
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