Abstract
The SNF2 gene family consists of a large group of proteins involved in transcriptional regulation, maintenance of chromosome integrity, and various aspects of DNA repair. We cloned a novel SNF2 family human cDNA, with sequence identity to the Escherichia coli RNA polymerase-binding protein HepA and named the human hepA-related protein (HHARP/SMARCAL1). In addition, the mouse ortholog (Mharp/Smarcal1) was cloned, and the Caenorhabditis elegans ortholog (CEHARP) was identified in the GenBank database. Phylogenetic analysis indicates that the HARP proteins share a high level of sequence similarity to the seven motif helicase core region (SNF2 domain) with identifiable orthologs in other eukaryotic species, except for yeast. Purified His-tagged HARP/SMARCAL1 protein exhibits single-stranded DNA-dependent ATPase activity, consistent with it being a member of the SNF2 family of proteins. Both the human and the mouse genes consist of 17 exons and 16 introns. The human gene maps to chromosome 2q34-q36, and the mouse gene is localized to the syntenic region of chromosome 1 (between markers Gls and Acrg). HARP/SMARCAL1 transcripts are ubiquitously expressed in human and mouse tissues, with testis presenting the highest levels of mRNA expression in humans.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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ATP-Binding Cassette Transporters*
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Adenosine Triphosphatases / metabolism
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Amino Acid Motifs
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Amino Acid Sequence
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Animals
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Chromosome Mapping
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Cloning, Molecular
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DNA Helicases / chemistry
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DNA Helicases / genetics*
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DNA Helicases / metabolism
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DNA, Complementary / genetics
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Humans
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Mice
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Molecular Sequence Data
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Nuclear Proteins*
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Phylogeny
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Prokaryotic Cells
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Protein Structure, Tertiary
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RNA, Messenger / metabolism
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Sequence Alignment
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors / metabolism
Substances
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ATP-Binding Cassette Transporters
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Bacterial Proteins
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DNA, Complementary
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DNA-Binding Proteins
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Nuclear Proteins
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RNA, Messenger
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SMARCA1 protein, human
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SMARCA2 protein, human
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Smarca2 protein, mouse
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Transcription Factors
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hepA protein, Anabaena
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SMARCAL1 protein, human
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Smarcal1 protein, mouse
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Adenosine Triphosphatases
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SMARCA4 protein, human
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Smarca4 protein, mouse
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DNA Helicases
Associated data
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GENBANK/AF082179
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GENBANK/AF209773
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GENBANK/AF210833
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GENBANK/AF210834
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GENBANK/AF210835
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GENBANK/AF210836
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GENBANK/AF210837
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GENBANK/AF210838
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GENBANK/AF210839
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GENBANK/AF210840
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GENBANK/AF210841
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GENBANK/AF210842