Interaction between the transcription factor SPBP and the positive cofactor RNF4. An interplay between protein binding zinc fingers

C Lyngsø; G Bouteiller; C K Damgaard; D Ryom; S Sanchez-Muñoz; P L Nørby; B J Bonven; P Jørgensen

doi:10.1074/jbc.M003405200

Interaction between the transcription factor SPBP and the positive cofactor RNF4. An interplay between protein binding zinc fingers

J Biol Chem. 2000 Aug 25;275(34):26144-9. doi: 10.1074/jbc.M003405200.

Authors

C Lyngsø¹, G Bouteiller, C K Damgaard, D Ryom, S Sanchez-Muñoz, P L Nørby, B J Bonven, P Jørgensen

Affiliation

¹ Department of Molecular and Structural Biology, University of Aarhus, C. F. Mollers Alle 130, DK-8000 Aarhus C., Denmark.

PMID: 10849425
DOI: 10.1074/jbc.M003405200

Abstract

The activator of stromelysin 1 gene transcription, SPBP, interacts with the RING finger protein RNF4. Both proteins are ubiquitously expressed and localized in the nucleus. RNF4 facilitates accumulation of specific SPBP-DNA complexes in vitro and acts as a positive cofactor in SPBP-mediated transactivation. SPBP harbors an internal zinc finger of the PHD/LAP type. This domain can form intra-chain protein-protein contacts in SPBP resulting in negative modulation of SPBP-RNF4 interaction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3 Cells
Amino Acid Sequence
Animals
Cell Nucleus / metabolism
DNA-Binding Proteins / metabolism
Escherichia coli
Fibroblasts / metabolism
Humans
Matrix Metalloproteinase 3 / genetics
Mice
Molecular Sequence Data
Nuclear Proteins*
Peptide Mapping
Protein Binding
Saccharomyces cerevisiae
Transcription Factors / metabolism*
Transcription, Genetic
Transcriptional Activation
Ubiquitin-Protein Ligases
Zinc Fingers*

Substances

DNA-Binding Proteins
Nuclear Proteins
RNF4 protein, human
TCF20 protein, human
Transcription Factors
Rnf4 protein, mouse
Ubiquitin-Protein Ligases
Matrix Metalloproteinase 3