Abstract
We have cloned human cDNA encoding a novel protein of 782 amino acids that contains the lipase consensus sequence Gly-Xaa-Ser-Xaa-Gly and several stretches surrounding the motif, which are homologous to those of the catalytic domain of cytosolic calcium-independent phospholipase A(2) (iPLA(2)). When expressed in COS-7 cells, the protein predominantly exists in the membrane fraction and exhibits a phospholipase A(2) activity in a calcium-independent manner. The transcript of the membrane-bound iPLA(2) gene is ubiquitously observed as a single band of approximately 3.3 kb on Northern blot, with the most abundant expression in the skeletal muscle and heart. By a search of the database, we have also identified its putative C. elegans homologue, which shows 47% identity with that of human in the iPLA(2) catalytic region. Thus the novel type of iPLA(2) is evolutionarily well conserved, suggestive of its biological significance.
Copyright 2000 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Amino Acid Sequence
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Animals
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Arachidonic Acid / metabolism
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Base Sequence
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COS Cells
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Caenorhabditis elegans / enzymology
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Caenorhabditis elegans / genetics
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Calcium Chloride / pharmacology
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Catalytic Domain
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Cell Membrane / drug effects
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Cell Membrane / enzymology*
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Cell Membrane / metabolism
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Cloning, Molecular
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Expressed Sequence Tags
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Group VI Phospholipases A2
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Helminth Proteins / chemistry
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Helminth Proteins / genetics
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Helminth Proteins / metabolism
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Humans
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Membrane Proteins / chemistry
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism*
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Molecular Sequence Data
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Phospholipases A / chemistry
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Phospholipases A / genetics*
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Phospholipases A / metabolism*
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transfection
Substances
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Helminth Proteins
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Membrane Proteins
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RNA, Messenger
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Recombinant Fusion Proteins
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Arachidonic Acid
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Phospholipases A
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Group VI Phospholipases A2
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PLA2G6 protein, human
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Calcium Chloride