A novel intracellular membrane-bound calcium-independent phospholipase A(2)

Biochem Biophys Res Commun. 2000 Jun 7;272(2):320-6. doi: 10.1006/bbrc.2000.2776.

Abstract

We have cloned human cDNA encoding a novel protein of 782 amino acids that contains the lipase consensus sequence Gly-Xaa-Ser-Xaa-Gly and several stretches surrounding the motif, which are homologous to those of the catalytic domain of cytosolic calcium-independent phospholipase A(2) (iPLA(2)). When expressed in COS-7 cells, the protein predominantly exists in the membrane fraction and exhibits a phospholipase A(2) activity in a calcium-independent manner. The transcript of the membrane-bound iPLA(2) gene is ubiquitously observed as a single band of approximately 3.3 kb on Northern blot, with the most abundant expression in the skeletal muscle and heart. By a search of the database, we have also identified its putative C. elegans homologue, which shows 47% identity with that of human in the iPLA(2) catalytic region. Thus the novel type of iPLA(2) is evolutionarily well conserved, suggestive of its biological significance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Arachidonic Acid / metabolism
  • Base Sequence
  • COS Cells
  • Caenorhabditis elegans / enzymology
  • Caenorhabditis elegans / genetics
  • Calcium Chloride / pharmacology
  • Catalytic Domain
  • Cell Membrane / drug effects
  • Cell Membrane / enzymology*
  • Cell Membrane / metabolism
  • Cloning, Molecular
  • Expressed Sequence Tags
  • Group VI Phospholipases A2
  • Helminth Proteins / chemistry
  • Helminth Proteins / genetics
  • Helminth Proteins / metabolism
  • Humans
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Phospholipases A / chemistry
  • Phospholipases A / genetics*
  • Phospholipases A / metabolism*
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transfection

Substances

  • Helminth Proteins
  • Membrane Proteins
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Arachidonic Acid
  • Phospholipases A
  • Group VI Phospholipases A2
  • PLA2G6 protein, human
  • Calcium Chloride

Associated data

  • GENBANK/AB041261