Characterization of a new TSPY gene family member in Yq (TSPYq1)

Cytogenet Cell Genet. 2000;88(1-2):159-62. doi: 10.1159/000015510.

Abstract

We investigated subinterval 6E on the human Y chromosome, a region frequently deleted in infertile males. YAC yOX17, mapped within subinterval 6E by STS-PCR, was analyzed for the presence of new genes. TSPYq1, a member of the TSPY multi-copy gene family, was isolated and characterized from a yOX17 cosmid subclone. PCR and FISH analysis performed on normal subjects and on patients with microdeletions of Yq suggested the presence of multiple copies of TSPY in Yq.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution / genetics
  • Base Sequence
  • Cell Cycle Proteins
  • Chromosome Deletion
  • Chromosomes, Artificial, Yeast / genetics
  • Cloning, Molecular
  • Cosmids / genetics
  • DNA-Binding Proteins / genetics*
  • Exons / genetics
  • Gene Dosage
  • Humans
  • In Situ Hybridization, Fluorescence
  • Infertility, Male / genetics
  • Male
  • Molecular Sequence Data
  • Multigene Family / genetics*
  • Nuclear Proteins*
  • Physical Chromosome Mapping
  • Polymerase Chain Reaction
  • Sequence Alignment
  • Sex-Determining Region Y Protein
  • Spermatogenesis / genetics
  • Transcription Factors*
  • Y Chromosome / genetics*

Substances

  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Nuclear Proteins
  • Sex-Determining Region Y Protein
  • TSPY1 protein, human
  • Transcription Factors

Associated data

  • GENBANK/AF106331

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