ROG, repressor of GATA, regulates the expression of cytokine genes

Immunity. 2000 Mar;12(3):323-33. doi: 10.1016/s1074-7613(00)80185-5.

Abstract

GATA-3 is a T cell-specific transcription factor and is essential for the development of the T cell lineage. Recently, it was shown that the expression of GATA-3 is further induced in CD4+ helper T cells upon differentiation into type 2 but not type 1 effector cells. Here, we report the molecular cloning of a GATA-3 interacting protein, repressor of GATA (ROG). ROG is a lymphoid-specific gene and is rapidly induced in Th cells upon stimulation with anti-CD3. In in vitro assays, ROG represses the GATA-3-induced transactivation. Furthermore, overexpression of ROG in Th clones inhibits the production of Th cytokines. Taken together, our results suggest that ROG might play a critical role in regulating the differentiation and activation of Th cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Chromosome Mapping
  • Cloning, Molecular
  • Cytokines / biosynthesis
  • Cytokines / genetics*
  • DNA / metabolism
  • DNA, Complementary
  • DNA-Binding Proteins / antagonists & inhibitors
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • GATA3 Transcription Factor
  • Gene Expression Regulation*
  • Humans
  • Jurkat Cells
  • Kruppel-Like Transcription Factors
  • Lymphocytes / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Precipitin Tests
  • Promyelocytic Leukemia Zinc Finger Protein
  • Protein Binding
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • Th1 Cells / metabolism
  • Th2 Cells / metabolism
  • Trans-Activators / antagonists & inhibitors
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Transcription Factors / genetics
  • Transcriptional Activation
  • Tumor Cells, Cultured
  • Zinc Fingers

Substances

  • Cytokines
  • DNA, Complementary
  • DNA-Binding Proteins
  • GATA3 Transcription Factor
  • GATA3 protein, human
  • Gata3 protein, mouse
  • Kruppel-Like Transcription Factors
  • Promyelocytic Leukemia Zinc Finger Protein
  • Repressor Proteins
  • Rog protein, mouse
  • Trans-Activators
  • Transcription Factors
  • ZBTB32 protein, human
  • Zbtb16 protein, mouse
  • ZBTB16 protein, human
  • DNA

Associated data

  • GENBANK/AF241232