Ermap, a gene coding for a novel erythroid specific adhesion/receptor membrane protein

Gene. 2000 Jan 25;242(1-2):337-45. doi: 10.1016/s0378-1119(99)00516-8.

Abstract

Ermap (erythroid membrane-associated protein), a gene coding for a novel transmembrane protein produced exclusively in erythroid cells, is described. It is mapped to murine Chromosome 4, 57 cM distal to the centromere. The initial cDNA clone was isolated from a day 9 murine embryonic erythroid cell cDNA library. The predicted peptide sequence suggests that ERMAP is a transmembrane protein with two extracellular immunoglobulin folds, as well as a highly conserved B30.2 domain and several phosphorylation consensus sequences in the cytoplasmic region. ERMAP shares a high homology throughout the entire peptide with butyrophilin, a glycoprotein essential for milk lipid droplet formation and release. A GFP-ERMAP fusion protein was localized to the plasma membrane and cytoplasmic vesicles in transiently transfected 293T cells. Northern blot analysis and in-situ hybridization demonstrated that Ermap expression was restricted to fetal and adult erythroid tissues. ERMAP is likely a novel adhesion/receptor molecule specific for erythroid cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blood Group Antigens
  • Blotting, Northern
  • Butyrophilins
  • Cell Adhesion
  • Cell Adhesion Molecules / genetics*
  • Cell Line
  • Cell Nucleus / chemistry
  • Chromosome Mapping
  • Cloning, Molecular
  • Cytoplasm / chemistry
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Embryo, Mammalian / metabolism
  • Erythrocytes / cytology
  • Erythrocytes / metabolism*
  • Gene Expression Regulation
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins
  • Humans
  • In Situ Hybridization
  • K562 Cells
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Male
  • Membrane Proteins / genetics*
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Muridae
  • RNA / genetics
  • RNA / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tissue Distribution

Substances

  • Blood Group Antigens
  • Butyrophilins
  • Cell Adhesion Molecules
  • DNA, Complementary
  • Ermap protein, mouse
  • Luminescent Proteins
  • Membrane Proteins
  • Recombinant Fusion Proteins
  • scianna antigens
  • Green Fluorescent Proteins
  • RNA

Associated data

  • GENBANK/AF153906