Abstract
Ermap (erythroid membrane-associated protein), a gene coding for a novel transmembrane protein produced exclusively in erythroid cells, is described. It is mapped to murine Chromosome 4, 57 cM distal to the centromere. The initial cDNA clone was isolated from a day 9 murine embryonic erythroid cell cDNA library. The predicted peptide sequence suggests that ERMAP is a transmembrane protein with two extracellular immunoglobulin folds, as well as a highly conserved B30.2 domain and several phosphorylation consensus sequences in the cytoplasmic region. ERMAP shares a high homology throughout the entire peptide with butyrophilin, a glycoprotein essential for milk lipid droplet formation and release. A GFP-ERMAP fusion protein was localized to the plasma membrane and cytoplasmic vesicles in transiently transfected 293T cells. Northern blot analysis and in-situ hybridization demonstrated that Ermap expression was restricted to fetal and adult erythroid tissues. ERMAP is likely a novel adhesion/receptor molecule specific for erythroid cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Blood Group Antigens
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Blotting, Northern
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Butyrophilins
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Cell Adhesion
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Cell Adhesion Molecules / genetics*
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Cell Line
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Cell Nucleus / chemistry
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Chromosome Mapping
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Cloning, Molecular
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Cytoplasm / chemistry
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Embryo, Mammalian / metabolism
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Erythrocytes / cytology
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Erythrocytes / metabolism*
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Gene Expression Regulation
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Gene Expression Regulation, Developmental
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Green Fluorescent Proteins
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Humans
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In Situ Hybridization
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K562 Cells
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Male
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Membrane Proteins / genetics*
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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Muridae
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RNA / genetics
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RNA / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Tissue Distribution
Substances
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Blood Group Antigens
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Butyrophilins
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Cell Adhesion Molecules
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DNA, Complementary
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Ermap protein, mouse
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Luminescent Proteins
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Membrane Proteins
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Recombinant Fusion Proteins
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scianna antigens
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Green Fluorescent Proteins
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RNA